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Assay Detail

CHEMBL1012738

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NECA from human cloned adenosine A2A receptor expressed in CHO cells
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Pyrido[2,3-e]-1,2,4-triazolo[4,3-a]pyrazin-1-one as a new scaffold to develop potent and selective human A3 adenosine receptor antagonists. Synthesis, pharmacological evaluation, and ligand-receptor modeling studies.
Colotta V, Lenzi O, Catarzi D, Varano F, Filacchioni G, Martini C, Trincavelli L, Ciampi O, Pugliese AM, Traini C, Pedata F, Morizzo E, Moro S.
J Med Chem (2009) 52 : 2407 -2419
PubMed 19301821 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 6.23 6.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL473648 1 6.68
CHEMBL473649 1 6.68
CHEMBL473839 1 6.68
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] 1 6.58
CHEMBL473650 1 5.65
CHEMBL1355736 THEOPHYLLINE 4.0 1 5.10

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL473648 Ki = 211.0 nM 6.68
CHEMBL473649 Ki = 208.0 nM 6.68
CHEMBL473839 Ki = 208.0 nM 6.68
CHEMBL183 1,3-DIPROPYL-8-CYCLOPENTYLXANTHINE [DPCPX] Ki = 260.0 nM 6.58
CHEMBL473650 Ki = 2240.0 nM 5.65
CHEMBL1355736 THEOPHYLLINE Ki = 7900.0 nM 5.10