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Assay Detail

CHEMBL1018653

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of FLAG-tagged full length human 11beta-HSD1 expressed in baculovirus-infected Trichoplusia ni Hi5 cells assessed as reduction of [3H]cortisone to [3H]cortisol by scintillation proximity assay in presence of NADPH
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BTI-TN-5B1-4
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

11-beta-hydroxysteroid dehydrogenase 1 (CHEMBL4235)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimization of novel di-substituted cyclohexylbenzamide derivatives as potent 11 beta-HSD1 inhibitors.
McMinn DL, Rew Y, Sudom A, Caille S, Degraffenreid M, He X, Hungate R, Jiang B, Jaen J, Julian LD, Kaizerman J, Novak P, Sun D, Tu H, Ursu S, Walker NP, Yan X, Ye Q, Wang Z, Powers JP.
Bioorg Med Chem Lett (2009) 19 : 1446 -1450
PubMed 19185488 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 8.11 9.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL511828 1 9.12
CHEMBL469699 1 8.89
CHEMBL518644 1 8.82
CHEMBL455443 1 8.80
CHEMBL2021495 1 8.80
CHEMBL2021496 1 8.80
CHEMBL452587 1 8.77
CHEMBL517760 1 8.77
CHEMBL2021499 1 8.48
CHEMBL473347 1 8.38
CHEMBL463031 1 8.31
CHEMBL515717 1 8.24
CHEMBL460933 1 8.05
CHEMBL2021500 1 7.96
CHEMBL526305 1 7.85
CHEMBL2021497 1 7.77
CHEMBL2021498 1 7.66
CHEMBL462154 1 7.58
CHEMBL517434 1 7.47
CHEMBL460923 1 7.37
CHEMBL460924 1 7.19
CHEMBL516674 1 6.82
CHEMBL463225 1 6.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL511828 IC50 = 0.76 nM 9.12
CHEMBL469699 IC50 = 1.3 nM 8.89
CHEMBL518644 IC50 = 1.5 nM 8.82
CHEMBL455443 IC50 = 1.6 nM 8.80
CHEMBL2021495 IC50 = 1.6 nM 8.80
CHEMBL2021496 IC50 = 1.6 nM 8.80
CHEMBL452587 IC50 = 1.7 nM 8.77
CHEMBL517760 IC50 = 1.7 nM 8.77
CHEMBL2021499 IC50 = 3.3 nM 8.48
CHEMBL473347 IC50 = 4.2 nM 8.38
CHEMBL463031 IC50 = 4.9 nM 8.31
CHEMBL515717 IC50 = 5.8 nM 8.24
CHEMBL460933 IC50 = 9.0 nM 8.05
CHEMBL2021500 IC50 = 11.0 nM 7.96
CHEMBL526305 IC50 = 14.0 nM 7.85
CHEMBL2021497 IC50 = 17.0 nM 7.77
CHEMBL2021498 IC50 = 22.0 nM 7.66
CHEMBL462154 IC50 = 26.0 nM 7.58
CHEMBL517434 IC50 = 34.0 nM 7.47
CHEMBL460923 IC50 = 43.0 nM 7.37
CHEMBL460924 IC50 = 64.0 nM 7.19
CHEMBL516674 IC50 = 150.0 nM 6.82
CHEMBL463225 IC50 = 190.0 nM 6.72