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Assay Detail

CHEMBL1026636

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat liver dihydrofolate reductase
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL2363)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

N9-substituted 2,4-diaminoquinazolines: synthesis and biological evaluation of lipophilic inhibitors of pneumocystis carinii and toxoplasma gondii dihydrofolate reductase.
Gangjee A, Adair OO, Pagley M, Queener SF.
J Med Chem (2008) 51 : 6195 -6200
PubMed 18771252 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 7.70 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL119 TRIMETREXATE 4.0 1 8.52
CHEMBL493717 1 8.39
CHEMBL7492 PIRITREXIM 2.0 1 8.36
CHEMBL521672 1 8.32
CHEMBL492895 1 8.28
CHEMBL493718 1 8.19
CHEMBL523004 1 8.02
CHEMBL522678 1 7.84
CHEMBL455012 1 7.80
CHEMBL492896 1 7.77
CHEMBL522543 1 7.72
CHEMBL325434 1 7.58
CHEMBL32113 1 7.24
CHEMBL32708 1 6.46
CHEMBL116816 1 4.96
CHEMBL22 TRIMETHOPRIM 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL119 TRIMETREXATE IC50 = 3.0 nM 8.52
CHEMBL493717 IC50 = 4.1 nM 8.39
CHEMBL7492 PIRITREXIM IC50 = 4.4 nM 8.36
CHEMBL521672 IC50 = 4.8 nM 8.32
CHEMBL492895 IC50 = 5.3 nM 8.28
CHEMBL493718 IC50 = 6.5 nM 8.19
CHEMBL523004 IC50 = 9.6 nM 8.02
CHEMBL522678 IC50 = 14.5 nM 7.84
CHEMBL455012 IC50 = 16.0 nM 7.80
CHEMBL492896 IC50 = 17.0 nM 7.77
CHEMBL522543 IC50 = 19.0 nM 7.72
CHEMBL325434 IC50 = 26.0 nM 7.58
CHEMBL32113 IC50 = 57.0 nM 7.24
CHEMBL32708 IC50 = 350.0 nM 6.46
CHEMBL116816 IC50 = 11000.0 nM 4.96
CHEMBL22 TRIMETHOPRIM IC50 = 133000.0 nM -