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Assay Detail

CHEMBL1031015

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant SMO expressed in mouse C3H10T1/2 cells assessed as inhibition of association of BODIPY-cyclopamine
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type C3H 10T1/2
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Protein smoothened (CHEMBL5971)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of a potent and orally active hedgehog pathway antagonist (IPI-926).
Tremblay MR, Lescarbeau A, Grogan MJ, Tan E, Lin G, Austad BC, Yu LC, Behnke ML, Nair SJ, Hagel M, White K, Conley J, Manna JD, Alvarez-Diez TM, Hoyt J, Woodward CN, Sydor JR, Pink M, MacDougall J, Campbell MJ, Cushing J, Ferguson J, Curtis MS, McGovern K, Read MA, Palombella VJ, Adams J, Castro AC.
J Med Chem (2009) 52 : 4400 -4418
PubMed 19522463 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 8.85 8.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL538867 PATIDEGIB 3.0 1 8.85

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL538867 PATIDEGIB IC50 = 1.4 nM 8.85