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Assay Detail

CHEMBL1045774

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human HCT116 cells assessed as reduction in cell number after 96 hrs by MTT assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HCT-116
Confidence 1 — Organism
Curated By Autocuration

Target

HCT-116 (CHEMBL394)
Type CELL-LINE
Organism Homo sapiens

Publication

Synthesis and biological evaluation of coumarin-based inhibitors of NAD(P)H: quinone oxidoreductase-1 (NQO1).
Nolan KA, Doncaster JR, Dunstan MS, Scott KA, Frenkel AD, Siegel D, Ross D, Barnes J, Levy C, Leys D, Whitehead RC, Stratford IJ, Bryce RA.
J Med Chem (2009) 52 : 7142 -7156
PubMed 19877692 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 4.41 4.72

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1466 DICUMAROL 4.0 1 4.72
CHEMBL570570 1 4.58
CHEMBL571058 1 4.46
CHEMBL569640 1 4.46
CHEMBL569641 1 4.41
CHEMBL576081 1 4.40
CHEMBL583323 1 4.32
CHEMBL569169 1 4.25
CHEMBL571496 1 4.10
CHEMBL569627 1 -
CHEMBL570332 1 -
CHEMBL570349 1 -
CHEMBL573693 1 -
CHEMBL571059 1 -
CHEMBL571272 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1466 DICUMAROL IC50 = 19000.0 nM 4.72
CHEMBL570570 IC50 = 26000.0 nM 4.58
CHEMBL571058 IC50 = 35000.0 nM 4.46
CHEMBL569640 IC50 = 35000.0 nM 4.46
CHEMBL569641 IC50 = 39000.0 nM 4.41
CHEMBL576081 IC50 = 40000.0 nM 4.40
CHEMBL583323 IC50 = 48000.0 nM 4.32
CHEMBL569169 IC50 = 56000.0 nM 4.25
CHEMBL571496 IC50 = 80000.0 nM 4.10
CHEMBL569627 IC50 = 187000.0 nM -
CHEMBL570332 IC50 > 400000.0 nM -
CHEMBL570349 IC50 = 180000.0 nM -
CHEMBL573693 IC50 = 178000.0 nM -
CHEMBL571059 IC50 = 102000.0 nM -
CHEMBL571272 IC50 = 176000.0 nM -