Assay Detail
Binding
CHEMBL1053956
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of human carbonic anhydrase 5B by stopped flow CO2 hydration assay
4
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Intermediate |
Target
Carbonic anhydrase 5B, mitochondrial (CHEMBL3969) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
The protein tyrosine kinase inhibitors imatinib and nilotinib strongly inhibit several mammalian alpha-carbonic anhydrase isoforms.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 3 | 5.62 | 7.27 |
| IC50 | 1 | 4.75 | 4.75 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL20 | ACETAZOLAMIDE | 4.0 | 1 | 7.27 |
| CHEMBL255863 | NILOTINIB | 4.0 | 1 | 4.83 |
| CHEMBL941 | IMATINIB | 4.0 | 1 | 4.77 |
| CHEMBL560175 | — | — | 1 | 4.75 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL20 | ACETAZOLAMIDE | Ki | = | 54.0 | nM | 7.27 |
| CHEMBL255863 | NILOTINIB | Ki | = | 14920.0 | nM | 4.83 |
| CHEMBL941 | IMATINIB | Ki | = | 17005.0 | nM | 4.77 |
| CHEMBL560175 | — | IC50 | = | 17700.0 | nM | 4.75 |