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Assay Detail

CHEMBL1054355

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of dopachrome tautomerase activity of MIF in human THP1 cells
25
Total Activities
25
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type THP-1
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Macrophage migration inhibitory factor (CHEMBL2085)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Novel derivatives of ISO-1 as potent inhibitors of MIF biological function.
Balachandran S, Rodge A, Gadekar PK, Yadav VN, Kamath D, Chetrapal-Kunwar A, Bhatt P, Srinivasan S, Sharma S, Vishwakarma RA, Dagia NM.
Bioorg Med Chem Lett (2009) 19 : 4773 -4776
PubMed 19592246 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 25 4.65 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL554271 1 6.30
CHEMBL550268 1 5.70
CHEMBL559951 1 4.92
CHEMBL558731 1 4.82
CHEMBL554732 1 4.82
CHEMBL556512 1 4.72
CHEMBL564237 1 4.70
CHEMBL559258 1 4.60
CHEMBL549864 1 4.55
CHEMBL562483 1 4.54
CHEMBL556445 1 4.54
CHEMBL559125 1 4.52
CHEMBL559555 1 4.32
CHEMBL549389 1 4.31
CHEMBL563301 1 4.30
CHEMBL538172 1 4.22
CHEMBL565030 1 4.22
CHEMBL210858 1 4.16
CHEMBL553881 1 4.00
CHEMBL554783 1 -
CHEMBL538170 1 -
CHEMBL563186 1 -
CHEMBL557060 1 -
CHEMBL559497 1 -
CHEMBL552426 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL554271 IC50 = 500.0 nM 6.30
CHEMBL550268 IC50 = 2000.0 nM 5.70
CHEMBL559951 IC50 = 12000.0 nM 4.92
CHEMBL558731 IC50 = 15000.0 nM 4.82
CHEMBL554732 IC50 = 15000.0 nM 4.82
CHEMBL556512 IC50 = 19000.0 nM 4.72
CHEMBL564237 IC50 = 20000.0 nM 4.70
CHEMBL559258 IC50 = 25000.0 nM 4.60
CHEMBL549864 IC50 = 28000.0 nM 4.55
CHEMBL562483 IC50 = 29000.0 nM 4.54
CHEMBL556445 IC50 = 29000.0 nM 4.54
CHEMBL559125 IC50 = 30000.0 nM 4.52
CHEMBL559555 IC50 = 48000.0 nM 4.32
CHEMBL549389 IC50 = 49000.0 nM 4.31
CHEMBL563301 IC50 = 50000.0 nM 4.30
CHEMBL538172 IC50 = 60000.0 nM 4.22
CHEMBL565030 IC50 = 60000.0 nM 4.22
CHEMBL210858 IC50 = 69000.0 nM 4.16
CHEMBL553881 IC50 = 100000.0 nM 4.00
CHEMBL554783 IC50 > 100000.0 nM -
CHEMBL538170 IC50 > 100000.0 nM -
CHEMBL563186 IC50 > 100000.0 nM -
CHEMBL557060 IC50 > 100000.0 nM -
CHEMBL559497 IC50 > 100000.0 nM -
CHEMBL552426 IC50 > 100000.0 nM -