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Assay Detail

CHEMBL1059967

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human COX1 expressed in african green monkey COS cells assessed as inhibition of arachidonic acid-stimulated PGE2 production by enzyme immunoassay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type COS
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

Prostaglandin G/H synthase 1 (CHEMBL221)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Identification and optimisation of a novel series of pyrimidine based cyclooxygenase-2 (COX-2) inhibitors. Utilisation of a biotransformation approach.
Beswick PJ, Blackaby AP, Bountra C, Brown T, Browning K, Campbell IB, Corfield J, Gleave RJ, Guntrip SB, Hall RM, Hindley S, Lambeth PF, Lucas F, Mathews N, Naylor A, Player H, Price HS, Sidebottom PJ, Taylor NL, Webb G, Wiseman J.
Bioorg Med Chem Lett (2009) 19 : 4509 -4514
PubMed 19523822 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.17 6.06

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL42485 DUP-697 1 6.06
CHEMBL550069 1 5.53
CHEMBL556929 1 5.36
CHEMBL558230 1 5.18
CHEMBL559056 1 5.00
CHEMBL552145 1 4.53
CHEMBL274990 SC-58125 1 4.50
CHEMBL559952 1 -
CHEMBL554903 1 -
CHEMBL564962 1 -
CHEMBL556724 1 -
CHEMBL559422 1 -
CHEMBL562768 1 -
CHEMBL563455 1 -
CHEMBL561888 1 -
CHEMBL551749 1 -
CHEMBL549457 1 -
CHEMBL564087 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL42485 DUP-697 IC50 = 870.0 nM 6.06
CHEMBL550069 IC50 = 2969.0 nM 5.53
CHEMBL556929 IC50 = 4340.0 nM 5.36
CHEMBL558230 IC50 = 6541.0 nM 5.18
CHEMBL559056 IC50 = 10000.0 nM 5.00
CHEMBL552145 IC50 = 29371.0 nM 4.53
CHEMBL274990 SC-58125 IC50 = 32000.0 nM 4.50
CHEMBL559952 IC50 > 100000.0 nM -
CHEMBL554903 IC50 > 100000.0 nM -
CHEMBL564962 IC50 > 100000.0 nM -
CHEMBL556724 IC50 > 100000.0 nM -
CHEMBL559422 IC50 > 100000.0 nM -
CHEMBL562768 IC50 > 100000.0 nM -
CHEMBL563455 IC50 > 34000.0 nM -
CHEMBL561888 IC50 > 52124.0 nM -
CHEMBL551749 IC50 - -
CHEMBL549457 IC50 - -
CHEMBL564087 IC50 - -