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Assay Detail

CHEMBL1060394

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human 5HT1A receptor expressed in CHO cells assessed as stimulation of [35S]GTPgammaS binding
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Intermediate

Target

5-hydroxytryptamine receptor 1A (CHEMBL214)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, potency, and in vivo evaluation of 2-piperazin-1-ylquinoline analogues as dual serotonin reuptake inhibitors and serotonin 5-HT1A receptor antagonists.
Zhou D, Stack GP, Lo J, Failli AA, Evrard DA, Harrison BL, Hatzenbuhler NT, Tran M, Croce S, Yi S, Golembieski J, Hornby GA, Lai M, Lin Q, Schechter LE, Smith DL, Shilling AD, Huselton C, Mitchell P, Beyer CE, Andree TH.
J Med Chem (2009) 52 : 4955 -4959
PubMed 19719241 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 15 6.58 7.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL562248 1 7.25
CHEMBL564148 1 7.24
CHEMBL551909 1 7.16
CHEMBL551304 1 7.16
CHEMBL551964 1 6.99
CHEMBL551107 1 6.93
CHEMBL550700 1 6.81
CHEMBL559778 1 6.79
CHEMBL560170 1 6.75
CHEMBL556474 1 6.53
CHEMBL551105 1 6.23
CHEMBL559579 1 6.11
CHEMBL565234 1 5.72
CHEMBL550169 1 5.67
CHEMBL550576 1 5.31

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL562248 IC50 = 56.7 nM 7.25
CHEMBL564148 IC50 = 58.0 nM 7.24
CHEMBL551909 IC50 = 70.0 nM 7.16
CHEMBL551304 IC50 = 70.0 nM 7.16
CHEMBL551964 IC50 = 102.1 nM 6.99
CHEMBL551107 IC50 = 117.0 nM 6.93
CHEMBL550700 IC50 = 156.0 nM 6.81
CHEMBL559778 IC50 = 164.0 nM 6.79
CHEMBL560170 IC50 = 180.0 nM 6.75
CHEMBL556474 IC50 = 294.0 nM 6.53
CHEMBL551105 IC50 = 588.0 nM 6.23
CHEMBL559579 IC50 = 781.0 nM 6.11
CHEMBL565234 IC50 = 1899.0 nM 5.72
CHEMBL550169 IC50 = 2165.0 nM 5.67
CHEMBL550576 IC50 = 4870.0 nM 5.31