Assay Detail
Functional
CHEMBL1071304
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at rat muscarinic M1 expressed in CHO cells assessed as stimulation of calcium mobilization
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Cell Type | CHO |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M1 (CHEMBL276) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
Chemical lead optimization of a pan G(q) mAChR M(1), M(3), M(5) positive allosteric modulator (PAM) lead. Part I: Development of the first highly selective M(5) PAM.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 8 | 5.36 | 5.50 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL503735 | — | — | 1 | 5.50 |
| CHEMBL523685 | — | — | 1 | 5.21 |
| CHEMBL593506 | — | — | 1 | - |
| CHEMBL603445 | — | — | 1 | - |
| CHEMBL596289 | — | — | 1 | - |
| CHEMBL596509 | — | — | 1 | - |
| CHEMBL596510 | — | — | 1 | - |
| CHEMBL595403 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL503735 | — | EC50 | = | 3200.0 | nM | 5.50 |
| CHEMBL523685 | — | EC50 | = | 6100.0 | nM | 5.21 |
| CHEMBL593506 | — | EC50 | > | 30000.0 | nM | - |
| CHEMBL603445 | — | EC50 | > | 30000.0 | nM | - |
| CHEMBL596289 | — | EC50 | > | 30000.0 | nM | - |
| CHEMBL596509 | — | EC50 | > | 30000.0 | nM | - |
| CHEMBL596510 | — | EC50 | > | 30000.0 | nM | - |
| CHEMBL595403 | — | EC50 | > | 30000.0 | nM | - |