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Assay Detail

CHEMBL1071908

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Functional
Agonist activity at human muscarinic M3 expressed in CHO cells assessed as stimulation of calcium mobilization
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M3 (CHEMBL245)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chemical lead optimization of a pan G(q) mAChR M(1), M(3), M(5) positive allosteric modulator (PAM) lead. Part I: Development of the first highly selective M(5) PAM.
Bridges TM, Kennedy JP, Cho HP, Breininger ML, Gentry PR, Hopkins CR, Conn PJ, Lindsley CW.
Bioorg Med Chem Lett (2010) 20 : 558 -562
PubMed 20004578 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 5.43 5.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL503735 1 5.66
CHEMBL523685 1 5.19

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL503735 EC50 = 2200.0 nM 5.66
CHEMBL523685 EC50 = 6400.0 nM 5.19