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Assay Detail

CHEMBL1103663

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant p38alpha-mediated AFT2 phosphorylation after 1 hr by HTRF assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 14 (CHEMBL260)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and evaluation of 7-alkyl-1,5-bis-aryl-pyrazolopyridinones as highly potent, selective, and orally efficacious inhibitors of p38alpha mitogen-activated protein kinase.
Pettus LH, Wurz RP, Xu S, Herberich B, Henkle B, Liu Q, McBride HJ, Mu S, Plant MH, Saris CJ, Sherman L, Wong LM, Chmait S, Lee MR, Mohr C, Hsieh F, Tasker AS.
J Med Chem (2010) 53 : 2973 -2985
PubMed 20218619 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 8.64 9.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1089866 1 9.22
CHEMBL1091929 1 9.10
CHEMBL1089456 1 9.05
CHEMBL1092012 1 9.00
CHEMBL1091928 1 8.96
CHEMBL1089865 1 8.85
CHEMBL1091930 1 8.82
CHEMBL1092011 1 8.77
CHEMBL1089122 1 8.72
CHEMBL1089534 1 8.72
CHEMBL1089513 1 8.70
CHEMBL1089457 1 8.64
CHEMBL1091199 1 8.64
CHEMBL1091927 1 8.10
CHEMBL1093461 1 6.34

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1089866 Ki = 0.6 nM 9.22
CHEMBL1091929 Ki = 0.8 nM 9.10
CHEMBL1089456 Ki = 0.9 nM 9.05
CHEMBL1092012 Ki = 1.0 nM 9.00
CHEMBL1091928 Ki = 1.1 nM 8.96
CHEMBL1089865 Ki = 1.4 nM 8.85
CHEMBL1091930 Ki = 1.5 nM 8.82
CHEMBL1092011 Ki = 1.7 nM 8.77
CHEMBL1089122 Ki = 1.9 nM 8.72
CHEMBL1089534 Ki = 1.9 nM 8.72
CHEMBL1089513 Ki = 2.0 nM 8.70
CHEMBL1089457 Ki = 2.3 nM 8.64
CHEMBL1091199 Ki = 2.3 nM 8.64
CHEMBL1091927 Ki = 8.0 nM 8.10
CHEMBL1093461 Ki = 458.0 nM 6.34