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Assay Detail

CHEMBL1104101

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Functional
Agonist activity at human histamine H2 receptor expressed in Sf9 cells coexpressing GsalphaS protein by steady-state GTPase assay
16
Total Activities
15
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Sf9
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H2 receptor (CHEMBL1941)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and structure-activity relationships of cyanoguanidine-type and structurally related histamine H4 receptor agonists.
Igel P, Geyer R, Strasser A, Dove S, Seifert R, Buschauer A.
J Med Chem (2009) 52 : 6297 -6313
PubMed 19791743 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 13 5.54 6.42
pKb 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1078746 1 6.42
CHEMBL1078359 2 6.36
CHEMBL90 HISTAMINE 4.0 1 5.92
CHEMBL1078358 1 5.70
CHEMBL275443 4-METHYLHISTAMINE 1 5.54
CHEMBL1080772 1 5.41
CHEMBL1078529 1 5.14
CHEMBL1078212 1 5.05
CHEMBL1077596 1 4.92
CHEMBL1078747 1 4.92
CHEMBL1078210 1 4.85
CHEMBL1081318 1 -
CHEMBL1078357 1 -
CHEMBL1081319 1 -
CHEMBL1078105 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1078746 EC50 = 380.19 nM 6.42
CHEMBL1078359 EC50 = 436.52 nM 6.36
CHEMBL1078359 EC50 = 645.65 nM 6.19
CHEMBL90 HISTAMINE EC50 = 1202.26 nM 5.92
CHEMBL1078358 EC50 = 1995.26 nM 5.70
CHEMBL275443 4-METHYLHISTAMINE EC50 = 2884.03 nM 5.54
CHEMBL1080772 EC50 = 3890.45 nM 5.41
CHEMBL1078529 EC50 = 7244.36 nM 5.14
CHEMBL1078212 EC50 = 8912.51 nM 5.05
CHEMBL1077596 EC50 = 12022.64 nM 4.92
CHEMBL1078747 EC50 = 12022.64 nM 4.92
CHEMBL1078210 EC50 = 14125.38 nM 4.85
CHEMBL1081318 EC50 > 10000.0 nM -
CHEMBL1078357 pKb = 5.8 -
CHEMBL1081319 pKb < 5.0 -
CHEMBL1078105 pKb < 5.0 -