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Assay Detail

CHEMBL1105082

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of nNOS in HEK293T cells assessed as nitrite formation
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK-293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Nitric oxide synthase 1 (CHEMBL3568)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Potent and selective neuronal nitric oxide synthase inhibitors with improved cellular permeability.
Xue F, Fang J, Lewis WW, Martásek P, Roman LJ, Silverman RB.
Bioorg Med Chem Lett (2010) 20 : 554 -557
PubMed 19963381 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 4.92 5.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL446971 1 5.05
CHEMBL1086858 1 5.00
CHEMBL1086859 1 4.72

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL446971 IC50 = 9000.0 nM 5.05
CHEMBL1086858 IC50 = 10000.0 nM 5.00
CHEMBL1086859 IC50 = 19000.0 nM 4.72