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Assay Detail

CHEMBL1106396

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human liver fructose-1,6-bisphosphatase expressed in Escherichia coli BL21 (DE3) assessed as reduction of NADP+ to NADPH by phosphoglucose isomerase/glucose-6-phosphate dehydrogenase coupled assay
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fructose-1,6-bisphosphatase 1 (CHEMBL3975)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Designing inhibitors against fructose 1,6-bisphosphatase: exploring natural products for novel inhibitor scaffolds.
Heng S, Harris KM, Kantrowitz ER.
Eur J Med Chem (2010) 45 : 1478 -1484
PubMed 20116906 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 5.11 5.22

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1097227 1 5.22
CHEMBL1097226 1 5.09
CHEMBL752 ADENOSINE PHOSPHATE 4.0 1 5.01
CHEMBL367881 (+)-USNIC ACID 1 -
CHEMBL1096898 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1097227 IC50 = 6000.0 nM 5.22
CHEMBL1097226 IC50 = 8100.0 nM 5.09
CHEMBL752 ADENOSINE PHOSPHATE IC50 = 9800.0 nM 5.01
CHEMBL367881 (+)-USNIC ACID IC50 = 371000.0 nM -
CHEMBL1096898 IC50 - -