Assay Detail

CHEMBL1108252

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Functional

Cytotoxicity against human HT1080 cells after 72 hrs by WST-8 assay

ChEMBL ↗

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Target

HT-1080 (CHEMBL614580) ↗

Open DB target context

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Primary target HT-1080

CHEMBL614580

Anchored from Assay target.

Protein identity HT-1080

Disease frame Disease evidence pending

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Review focus Open DB context pending

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Total Activities

Compounds Tested

Activity Types

Assay Parameters

Assay Information

Assay Type	Functional
Organism	Homo sapiens
Cell Type	HT-1080
Confidence	1 — Organism
Curated By	Autocuration

Target

HT-1080 (CHEMBL614580) ↗

Type	CELL-LINE
Organism	Homo sapiens

Publication

Cucurbitacin E as a new inhibitor of cofilin phosphorylation in human leukemia U937 cells.

Nakashima S, Matsuda H, Kurume A, Oda Y, Nakamura S, Yamashita M, Yoshikawa M.
Bioorg Med Chem Lett (2010) 20 : 2994 -2997
PubMed 20347305 · DOI

Activity Statistics

Type	Count	Avg pChEMBL	Best pChEMBL
IC50	2	8.37	9.33

Compounds Tested

Compound	Name	Phase	Activities	Best pChEMBL
CHEMBL387737	CUCURBITACIN I	—	1	9.33
CHEMBL455056	CUCURBITACIN E	—	1	7.40

Activity Data

Compound	Name	Type	Rel.	Value	Units	pChEMBL
CHEMBL387737	CUCURBITACIN I	IC50	=	0.47	nM	9.33
CHEMBL455056	CUCURBITACIN E	IC50	=	40.0	nM	7.40