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Assay Detail

CHEMBL1109225

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human MDA-MB-231 cells after 3 days by MTT assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type MDA-MB-231
Confidence 1 — Organism
Curated By Autocuration

Target

MDA-MB-231 (CHEMBL400)
Type CELL-LINE
Organism Homo sapiens

Publication

Rational design and semisynthesis of betulinic acid analogues as potent topoisomerase inhibitors.
Bar FM, Khanfar MA, Elnagar AY, Liu H, Zaghloul AM, Badria FA, Sylvester PW, Ahmad KF, Raisch KP, El Sayed KA.
J Nat Prod (2009) 72 : 1643 -1650
PubMed 19691293 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 5.75 6.77

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1081516 1 6.77
CHEMBL1078125 1 6.35
CHEMBL1078318 1 6.19
CHEMBL1078319 1 6.10
CHEMBL1078316 1 6.04
CHEMBL1078221 1 5.86
CHEMBL567578 1 5.39
CHEMBL431525 BETUNOLIC ACID 1 5.01
CHEMBL1078315 1 5.01
CHEMBL269277 BETULINIC ACID 1.0 1 4.82
CHEMBL1080077 1 -
CHEMBL1080792 1 -
CHEMBL1078317 1 -
CHEMBL1078126 1 -
CHEMBL65 CAMPTOTHECIN -1.0 1 -
CHEMBL44657 ETOPOSIDE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1081516 IC50 = 170.0 nM 6.77
CHEMBL1078125 IC50 = 450.0 nM 6.35
CHEMBL1078318 IC50 = 650.0 nM 6.19
CHEMBL1078319 IC50 = 800.0 nM 6.10
CHEMBL1078316 IC50 = 920.0 nM 6.04
CHEMBL1078221 IC50 = 1370.0 nM 5.86
CHEMBL567578 IC50 = 4080.0 nM 5.39
CHEMBL431525 BETUNOLIC ACID IC50 = 9770.0 nM 5.01
CHEMBL1078315 IC50 = 9830.0 nM 5.01
CHEMBL269277 BETULINIC ACID IC50 = 15240.0 nM 4.82
CHEMBL1080077 IC50 > 25000.0 nM -
CHEMBL1080792 IC50 > 25000.0 nM -
CHEMBL1078317 IC50 > 25000.0 nM -
CHEMBL1078126 IC50 > 25000.0 nM -
CHEMBL65 CAMPTOTHECIN IC50 - -
CHEMBL44657 ETOPOSIDE IC50 - -