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Assay Detail

CHEMBL1114569

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiproliferative activity against human HT-29 cells after 72 hrs by sulforhodamine B assay
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HT-29
Confidence 1 — Organism
Curated By Autocuration

Target

HT-29 (CHEMBL384)
Type CELL-LINE
Organism Homo sapiens

Publication

A series of alpha-heterocyclic carboxaldehyde thiosemicarbazones inhibit topoisomerase IIalpha catalytic activity.
Huang H, Chen Q, Ku X, Meng L, Lin L, Wang X, Zhu C, Wang Y, Chen Z, Li M, Jiang H, Chen K, Ding J, Liu H.
J Med Chem (2010) 53 : 3048 -3064
PubMed 20353152 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 4.84 7.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1096926 1 7.70
CHEMBL1095909 1 7.40
CHEMBL1094270 1 6.60
CHEMBL1097930 1 6.39
CHEMBL1097928 1 6.35
CHEMBL1095908 1 4.94
CHEMBL1097929 1 4.89
CHEMBL1095906 1 4.87
CHEMBL1098923 1 4.81
CHEMBL1094607 1 4.77
CHEMBL1098955 1 4.74
CHEMBL1098924 1 4.68
CHEMBL1098604 1 4.54
CHEMBL1094910 1 4.37
CHEMBL1098927 1 4.32
CHEMBL1098954 1 4.26
CHEMBL1095907 1 4.24
CHEMBL1098953 1 4.23
CHEMBL1099291 1 4.20
CHEMBL1098603 1 4.13
CHEMBL1096925 1 4.12
CHEMBL1097947 1 4.08
CHEMBL1097946 1 4.08
CHEMBL1095271 1 4.07
CHEMBL1097257 1 4.01
CHEMBL1098270 1 4.00
CHEMBL215065 1 4.00
CHEMBL1094271 1 -
CHEMBL1094608 1 -
CHEMBL1098290 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1096926 IC50 = 20.0 nM 7.70
CHEMBL1095909 IC50 = 40.0 nM 7.40
CHEMBL1094270 IC50 = 250.0 nM 6.60
CHEMBL1097930 IC50 = 410.0 nM 6.39
CHEMBL1097928 IC50 = 450.0 nM 6.35
CHEMBL1095908 IC50 = 11520.0 nM 4.94
CHEMBL1097929 IC50 = 12830.0 nM 4.89
CHEMBL1095906 IC50 = 13380.0 nM 4.87
CHEMBL1098923 IC50 = 15550.0 nM 4.81
CHEMBL1094607 IC50 = 16990.0 nM 4.77
CHEMBL1098955 IC50 = 18110.0 nM 4.74
CHEMBL1098924 IC50 = 20680.0 nM 4.68
CHEMBL1098604 IC50 = 28500.0 nM 4.54
CHEMBL1094910 IC50 = 42250.0 nM 4.37
CHEMBL1098927 IC50 = 48100.0 nM 4.32
CHEMBL1098954 IC50 = 54350.0 nM 4.26
CHEMBL1095907 IC50 = 57500.0 nM 4.24
CHEMBL1098953 IC50 = 58940.0 nM 4.23
CHEMBL1099291 IC50 = 63880.0 nM 4.20
CHEMBL1098603 IC50 = 74710.0 nM 4.13
CHEMBL1096925 IC50 = 75650.0 nM 4.12
CHEMBL1097947 IC50 = 82290.0 nM 4.08
CHEMBL1097946 IC50 = 82350.0 nM 4.08
CHEMBL1095271 IC50 = 85530.0 nM 4.07
CHEMBL1097257 IC50 = 97180.0 nM 4.01
CHEMBL1098270 IC50 = 99160.0 nM 4.00
CHEMBL215065 IC50 = 100000.0 nM 4.00
CHEMBL1099252 IC50 > 100000.0 nM -
CHEMBL1097927 IC50 > 100000.0 nM -
CHEMBL1098925 IC50 > 100000.0 nM -
CHEMBL1098952 IC50 > 100000.0 nM -
CHEMBL1098602 IC50 > 100000.0 nM -
CHEMBL1098601 IC50 > 100000.0 nM -
CHEMBL1098290 IC50 > 100000.0 nM -
CHEMBL1094608 IC50 > 100000.0 nM -
CHEMBL1094271 IC50 > 100000.0 nM -