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Assay Detail

CHEMBL1114668

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at glucocorticoid receptor in human MDA-kb2 cells transfected with MMTV-LUC assessed as induction of MMTV-LTR/promoter linked LUC gene by luciferase transactivation assay
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type MDA-kb2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL2034)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

5-Functionalized indazoles as glucocorticoid receptor agonists.
Bai M, Carr G, Deorazio RJ, Friedrich TD, Dobritsa S, Fitzpatrick K, Guzzo PR, Kitchen DB, Lynch MA, Peace D, Sajad M, Usyatinsky A, Wolf MA.
Bioorg Med Chem Lett (2010) 20 : 3017 -3020
PubMed 20427184 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 6 6.56 7.26

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL131 PREDNISOLONE 4.0 1 7.26
CHEMBL1094208 1-(2,3-DICHLOROPHENYL)-2,2,2-TRIFLUORO-1-(1-(4-FLUOROPHENYL)-1H-INDAZOL-5-YL)ETHANOL (ENANTIOMERIC MIX) 1 6.46
CHEMBL1094231 1-(3-CHLORO-4-FLUOROPHENYL)-2,2,2-TRIFLUORO-1-(1-(4-FLUOROPHENYL)-1H-INDAZOL-5-YL)ETHANOL (ENANTIOMERIC MIX) 1 5.96
CHEMBL1094209 1 -
CHEMBL1096467 1 -
CHEMBL1094514 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL131 PREDNISOLONE EC50 = 55.0 nM 7.26
CHEMBL1094208 1-(2,3-DICHLOROPHENYL)-2,2,2-TRIFLUORO-1-(1-(4-FLUOROPHENYL)-1H-INDAZOL-5-YL)ETHANOL (ENANTIOMERIC MIX) EC50 = 350.0 nM 6.46
CHEMBL1094231 1-(3-CHLORO-4-FLUOROPHENYL)-2,2,2-TRIFLUORO-1-(1-(4-FLUOROPHENYL)-1H-INDAZOL-5-YL)ETHANOL (ENANTIOMERIC MIX) EC50 = 1100.0 nM 5.96
CHEMBL1094209 EC50 - -
CHEMBL1096467 EC50 - -
CHEMBL1094514 EC50 - -