Assay Detail
Functional
CHEMBL1115329
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at rat mGlu4 receptor expressed in HEK293 cells co-transfected with G-protein alpha and EAAC1 assessed as increase in intracellular calcium level after 1 hr by fluorescence microplate reader
13
Total Activities
13
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Rattus norvegicus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Metabotropic glutamate receptor 4 (CHEMBL2787) ↗| Type | SINGLE PROTEIN |
| Organism | Rattus norvegicus |
Publication
A virtual screening hit reveals new possibilities for developing group III metabotropic glutamate receptor agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 12 | 5.53 | 6.89 |
| Activity | 1 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL33567 | — | — | 1 | 6.89 |
| CHEMBL1092243 | — | — | 1 | 6.00 |
| CHEMBL1076865 | — | — | 1 | 6.00 |
| CHEMBL1092315 | — | — | 1 | 5.62 |
| CHEMBL1089515 | — | — | 1 | 5.21 |
| CHEMBL1092316 | — | — | 1 | 5.14 |
| CHEMBL1089852 | — | — | 1 | 5.13 |
| CHEMBL1093009 | — | — | 1 | 5.07 |
| CHEMBL1093612 | — | — | 1 | 4.70 |
| CHEMBL1092312 | — | — | 1 | - |
| CHEMBL1092314 | — | — | 1 | - |
| CHEMBL1089174 | — | — | 1 | - |
| CHEMBL1089175 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL33567 | — | EC50 | = | 130.0 | nM | 6.89 |
| CHEMBL1092243 | — | EC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL1076865 | — | EC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL1092315 | — | EC50 | = | 2400.0 | nM | 5.62 |
| CHEMBL1089515 | — | EC50 | = | 6150.0 | nM | 5.21 |
| CHEMBL1092316 | — | EC50 | = | 7200.0 | nM | 5.14 |
| CHEMBL1089852 | — | EC50 | = | 7400.0 | nM | 5.13 |
| CHEMBL1093009 | — | EC50 | = | 8500.0 | nM | 5.07 |
| CHEMBL1093612 | — | EC50 | = | 20000.0 | nM | 4.70 |
| CHEMBL1092312 | — | EC50 | = | 141000.0 | nM | - |
| CHEMBL1092314 | — | EC50 | = | 123000.0 | nM | - |
| CHEMBL1089174 | — | EC50 | = | 209000.0 | nM | - |
| CHEMBL1089175 | — | Activity | - | — | - |