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Assay Detail

CHEMBL1116652

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR in human A431 cells by HTRF assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type A-431
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of pyrrolopyridazine derivatives as novel HER-2 tyrosine kinase inhibitors.
Tang PC, Feng J, Huang L, Xu Z, Cheng L, Zhang X, Zhang L, Hu B.
Bioorg Med Chem Lett (2009) 19 : 6437 -6440
PubMed 19815412 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 5.76 6.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1201179 LAPATINIB DITOSYLATE 4.0 1 6.85
CHEMBL1082012 1 6.59
CHEMBL1079742 ERLOTINIB HYDROCHLORIDE 4.0 1 6.38
CHEMBL1080812 1 6.06
CHEMBL1081849 1 6.01
CHEMBL1087368 1 5.94
CHEMBL1080990 1 5.92
CHEMBL1080506 1 4.90
CHEMBL1080272 1 4.82
CHEMBL1080271 1 4.11

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1201179 LAPATINIB DITOSYLATE IC50 = 140.0 nM 6.85
CHEMBL1082012 IC50 = 257.0 nM 6.59
CHEMBL1079742 ERLOTINIB HYDROCHLORIDE IC50 = 420.0 nM 6.38
CHEMBL1080812 IC50 = 867.0 nM 6.06
CHEMBL1081849 IC50 = 980.0 nM 6.01
CHEMBL1087368 IC50 = 1160.0 nM 5.94
CHEMBL1080990 IC50 = 1210.0 nM 5.92
CHEMBL1080506 IC50 = 12650.0 nM 4.90
CHEMBL1080272 IC50 = 15140.0 nM 4.82
CHEMBL1080271 IC50 = 77220.0 nM 4.11