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Assay Detail

CHEMBL1120072

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of 8-anilino-1-naphthalene-sulfonic acid from human His-FABP4 expressed in Escherichia coli BL21 (DE3) cells after 3 mins
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BL21-DE3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Fatty acid-binding protein, adipocyte (CHEMBL2083)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of highly selective inhibitors of human fatty acid binding protein 4 (FABP4) by virtual screening.
Cai H, Yan G, Zhang X, Gorbenko O, Wang H, Zhu W.
Bioorg Med Chem Lett (2010) 20 : 3675 -3679
PubMed 20471252 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 4.53 4.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1085570 1 4.87
CHEMBL267476 LINOLEIC ACID 2.0 1 4.87
CHEMBL8659 OLEIC ACID 2.0 1 4.58
CHEMBL1085571 1 4.38
CHEMBL1085797 1 4.25
CHEMBL1086224 1 4.22
CHEMBL1084821 1 -
CHEMBL1086457 1 -
CHEMBL1085065 1 -
CHEMBL1084578 1 -
CHEMBL1084579 1 -
CHEMBL1084580 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1085570 IC50 = 13500.0 nM 4.87
CHEMBL267476 LINOLEIC ACID IC50 = 13600.0 nM 4.87
CHEMBL8659 OLEIC ACID IC50 = 26100.0 nM 4.58
CHEMBL1085571 IC50 = 42200.0 nM 4.38
CHEMBL1085797 IC50 = 56300.0 nM 4.25
CHEMBL1086224 IC50 = 59500.0 nM 4.22
CHEMBL1084821 IC50 > 100000.0 nM -
CHEMBL1086457 IC50 > 100000.0 nM -
CHEMBL1085065 IC50 > 100000.0 nM -
CHEMBL1084578 IC50 > 100000.0 nM -
CHEMBL1084579 IC50 > 100000.0 nM -
CHEMBL1084580 IC50 > 100000.0 nM -