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Assay Detail

CHEMBL1120738

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]WIN-35428 from human recombinant DAT expressed in CHO cells
19
Total Activities
19
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent dopamine transporter (CHEMBL238)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel selective norepinephrine reuptake inhibitors: 4-[3-aryl-2,2-dioxido-2,1,3-benzothiadiazol-1(3H)-yl]-1-(methylamino)butan-2-ols (WYE-103231).
O'Neill DJ, Adedoyin A, Alfinito PD, Bray JA, Cosmi S, Deecher DC, Fensome A, Harrison J, Leventhal L, Mann C, McComas CC, Sullivan NR, Spangler TB, Uveges AJ, Trybulski EJ, Whiteside GT, Zhang P.
J Med Chem (2010) 53 : 4511 -4521
PubMed 20462211 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 5.90 6.58
Ki 1 7.66 7.66

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL781 MAZINDOL 4.0 1 7.66
CHEMBL1084828 1 6.58
CHEMBL1085322 1 6.57
CHEMBL1083430 1 6.54
CHEMBL1084829 1 6.41
CHEMBL1085338 1 6.29
CHEMBL1084313 1 6.29
CHEMBL1084311 1 6.00
CHEMBL1083392 1 5.83
CHEMBL1082292 1 5.73
CHEMBL1083118 1 5.68
CHEMBL1083409 1 5.67
CHEMBL1085066 1 5.63
CHEMBL1083410 1 5.62
CHEMBL1085323 1 5.59
CHEMBL1083429 1 5.58
CHEMBL1084312 1 5.50
CHEMBL1082458 1 5.44
CHEMBL1083428 1 5.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL781 MAZINDOL Ki = 22.1 nM 7.66
CHEMBL1084828 IC50 = 262.0 nM 6.58
CHEMBL1085322 IC50 = 267.0 nM 6.57
CHEMBL1083430 IC50 = 291.0 nM 6.54
CHEMBL1084829 IC50 = 386.0 nM 6.41
CHEMBL1085338 IC50 = 512.0 nM 6.29
CHEMBL1084313 IC50 = 509.0 nM 6.29
CHEMBL1084311 IC50 = 998.0 nM 6.00
CHEMBL1083392 IC50 = 1475.0 nM 5.83
CHEMBL1082292 IC50 = 1872.0 nM 5.73
CHEMBL1083118 IC50 = 2086.0 nM 5.68
CHEMBL1083409 IC50 = 2113.0 nM 5.67
CHEMBL1085066 IC50 = 2326.0 nM 5.63
CHEMBL1083410 IC50 = 2409.0 nM 5.62
CHEMBL1085323 IC50 = 2570.0 nM 5.59
CHEMBL1083429 IC50 = 2618.0 nM 5.58
CHEMBL1084312 IC50 = 3185.0 nM 5.50
CHEMBL1082458 IC50 = 3617.0 nM 5.44
CHEMBL1083428 IC50 = 5500.0 nM 5.26