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Assay Detail

CHEMBL1168403

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of (+/-)-[3H]epibatidine from alpha4beta2 nAChR in rat cortical membrane
13
Total Activities
13
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Neuronal acetylcholine receptor; alpha4/beta2 (CHEMBL1907596)
Type PROTEIN COMPLEX
Organism Rattus norvegicus

Publication

Novel tricyclic Delta(2)-isoxazoline and 3-oxo-2-methyl-isoxazolidine derivatives: synthesis and binding affinity at neuronal nicotinic acetylcholine receptor subtypes.
Dallanoce C, Frigerio F, Martelli G, Grazioso G, Matera C, Pomè DY, Pucci L, Clementi F, Gotti C, De Amici M.
Bioorg Med Chem (2010) 18 : 4498 -4508
PubMed 20478710 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 5.95 10.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL6623 EPIBATIDINE 1 10.46
CHEMBL497939 CYTISINICLINE 3.0 1 9.40
CHEMBL1164304 1 7.03
CHEMBL1163516 1 6.12
CHEMBL1163244 1 6.04
CHEMBL1164456 1 5.75
CHEMBL1163249 1 5.35
CHEMBL1794974 1 4.51
CHEMBL1164458 1 4.30
CHEMBL1163517 1 4.28
CHEMBL1163515 1 4.10
CHEMBL1164459 1 4.00
CHEMBL1164404 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL6623 EPIBATIDINE Ki = 0.035 nM 10.46
CHEMBL497939 CYTISINICLINE Ki = 0.4 nM 9.40
CHEMBL1164304 Ki = 94.0 nM 7.03
CHEMBL1163516 Ki = 750.0 nM 6.12
CHEMBL1163244 Ki = 920.0 nM 6.04
CHEMBL1164456 Ki = 1800.0 nM 5.75
CHEMBL1163249 Ki = 4500.0 nM 5.35
CHEMBL1794974 Ki = 31000.0 nM 4.51
CHEMBL1164458 Ki = 50000.0 nM 4.30
CHEMBL1163517 Ki = 53000.0 nM 4.28
CHEMBL1163515 Ki = 79000.0 nM 4.10
CHEMBL1164459 Ki = 100000.0 nM 4.00
CHEMBL1164404 Ki = 260000.0 nM -