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Assay Detail

CHEMBL1174254

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of N-terminal 6His-tagged human aldose reductase expressed in Escherichia coli BL21(DE3) mediated NADPH linked pyridine-3-aldehyde reduction
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Aldo-keto reductase family 1 member B1 (CHEMBL1900)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Chromene-3-carboxamide derivatives discovered from virtual screening as potent inhibitors of the tumour maker, AKR1B10.
Endo S, Matsunaga T, Kuwata K, Zhao HT, El-Kabbani O, Kitade Y, Hara A.
Bioorg Med Chem (2010) 18 : 2485 -2490
PubMed 20304656 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.48 8.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL436 TOLRESTAT 4.0 1 8.00
CHEMBL1172665 1 7.96
CHEMBL1171546 1 7.68
CHEMBL56337 EPALRESTAT 4.0 1 7.68
CHEMBL273910 MINALRESTAT 2.0 1 7.60
CHEMBL1169757 1 7.47
CHEMBL1169756 1 7.44
CHEMBL10372 ZOPOLRESTAT 2.0 1 7.22
CHEMBL266497 SORBINIL 3.0 1 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL436 TOLRESTAT IC50 = 10.0 nM 8.00
CHEMBL1172665 IC50 = 11.0 nM 7.96
CHEMBL1171546 IC50 = 21.0 nM 7.68
CHEMBL56337 EPALRESTAT IC50 = 21.0 nM 7.68
CHEMBL273910 MINALRESTAT IC50 = 25.0 nM 7.60
CHEMBL1169757 IC50 = 34.0 nM 7.47
CHEMBL1169756 IC50 = 36.0 nM 7.44
CHEMBL10372 ZOPOLRESTAT IC50 = 60.0 nM 7.22
CHEMBL266497 SORBINIL IC50 = 550.0 nM 6.26