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Assay Detail

CHEMBL1175732

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]DAMGO from rat mu opioid receptor expressed in mouse HN9.10 cells
26
Total Activities
13
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL270)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Design and synthesis of trivalent ligands targeting opioid, cholecystokinin, and melanocortin receptors for the treatment of pain.
Lee YS, Fernandes S, Kulkarani V, Mayorov A, Davis P, Ma SW, Brown K, Gillies RJ, Lai J, Porreca F, Hruby VJ.
Bioorg Med Chem Lett (2010) 20 : 4080 -4084
PubMed 20547453 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 13 7.80 9.89
IC50 13 7.46 9.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1172253 2 9.89
CHEMBL1172252 2 9.16
CHEMBL235743 2 8.55
CHEMBL1172428 2 8.31
CHEMBL1172248 2 8.23
CHEMBL1172245 2 8.13
CHEMBL1172247 2 8.07
CHEMBL1172251 2 7.85
CHEMBL1172249 2 7.75
CHEMBL2372623 2 7.33
CHEMBL1172429 2 6.60
CHEMBL1172244 2 5.91
CHEMBL1172246 2 5.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1172253 Ki = 0.13 nM 9.89
CHEMBL1172253 IC50 = 0.2512 nM 9.60
CHEMBL1172252 Ki = 0.69 nM 9.16
CHEMBL1172252 IC50 = 1.259 nM 8.90
CHEMBL235743 Ki = 2.8 nM 8.55
CHEMBL1172428 Ki = 4.9 nM 8.31
CHEMBL1172248 Ki = 5.9 nM 8.23
CHEMBL235743 IC50 = 6.31 nM 8.20
CHEMBL1172245 Ki = 7.4 nM 8.13
CHEMBL1172247 Ki = 8.6 nM 8.07
CHEMBL1172428 IC50 = 10.0 nM 8.00
CHEMBL1172248 IC50 = 12.59 nM 7.90
CHEMBL1172251 Ki = 14.0 nM 7.85
CHEMBL1172249 Ki = 18.0 nM 7.75
CHEMBL1172247 IC50 = 19.95 nM 7.70
CHEMBL1172245 IC50 = 31.62 nM 7.50
CHEMBL1172251 IC50 = 31.62 nM 7.50
CHEMBL1172249 IC50 = 39.81 nM 7.40
CHEMBL2372623 Ki = 47.0 nM 7.33
CHEMBL2372623 IC50 = 100.0 nM 7.00
CHEMBL1172429 Ki = 250.0 nM 6.60
CHEMBL1172429 IC50 = 501.19 nM 6.30
CHEMBL1172244 Ki = 1240.0 nM 5.91
CHEMBL1172246 Ki = 2100.0 nM 5.68
CHEMBL1172244 IC50 = 2511.89 nM 5.60
CHEMBL1172246 IC50 = 3981.07 nM 5.40