Assay Detail
Binding
CHEMBL1212349
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Inhibition of human recombinant GST-fused NIK expressed in Sf9 cells after 60 mins by radiometric protein kinase assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Mitogen-activated protein kinase kinase kinase 14 (CHEMBL5888) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
NF-kappaB inducing kinase (NIK) inhibitors: identification of new scaffolds using virtual screening.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 4.66 | 5.64 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL388978 | STAUROSPORINE | — | 1 | 5.64 |
| CHEMBL1210769 | — | — | 1 | 4.29 |
| CHEMBL1210770 | — | — | 1 | 4.05 |
| CHEMBL295316 | PLUMBAGIN | 1.0 | 1 | - |
| CHEMBL1210771 | — | — | 1 | - |
| CHEMBL1210772 | — | — | 1 | - |
| CHEMBL1210773 | — | — | 1 | - |
| CHEMBL1210774 | — | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL388978 | STAUROSPORINE | IC50 | = | 2300.0 | nM | 5.64 |
| CHEMBL1210769 | — | IC50 | = | 51000.0 | nM | 4.29 |
| CHEMBL1210770 | — | IC50 | = | 90000.0 | nM | 4.05 |
| CHEMBL295316 | PLUMBAGIN | IC50 | > | 100000.0 | nM | - |
| CHEMBL1210771 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL1210772 | — | IC50 | > | 100000.0 | nM | - |
| CHEMBL1210773 | — | IC50 | > | 50000.0 | nM | - |
| CHEMBL1210774 | — | IC50 | > | 100000.0 | nM | - |