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Assay Detail

CHEMBL1220906

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to N-terminal Shh by surface plasmon resonance assay
3
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Sonic hedgehog protein (CHEMBL5602)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A small molecule that binds Hedgehog and blocks its signaling in human cells.
Stanton BZ, Peng LF, Maloof N, Nakai K, Wang X, Duffner JL, Taveras KM, Hyman JM, Lee SW, Koehler AN, Chen JK, Fox JL, Mandinova A, Schreiber SL.
Nat Chem Biol (2009) 5 : 154 -156
PubMed 19151731 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 2 5.28 5.51
Activity 1 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL473392 ROBOTNIKININ 1 5.51
CHEMBL1213326 2 5.05

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL473392 ROBOTNIKININ Kd = 3100.0 nM 5.51
CHEMBL1213326 Kd = 9000.0 nM 5.05
CHEMBL1213326 Activity - -