Assay Detail
Functional
CHEMBL1228353
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Inhibition of protein synthesis in human HeLa cells assessed as [35S]cysteine/methionine utilization after 2 hrs by scintillation spectroscopy
12
Total Activities
8
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HeLa |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Inhibition | 6 | - | - |
| IC50 | 6 | 6.49 | 7.42 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1221911 | LACTIMIDOMYCIN | — | 2 | 7.42 |
| CHEMBL1222014 | 2H LACTIMIDOMYCIN K | — | 1 | 7.01 |
| CHEMBL1554 | DACTINOMYCIN | 4.0 | 2 | 6.28 |
| CHEMBL123292 | CYCLOHEXIMIDE | 2.0 | 2 | 6.27 |
| CHEMBL1221912 | ISOMIGRASTATIN | — | 2 | 6.21 |
| CHEMBL1222015 | DIDEHYDRO ISOMIGRASTATIN | — | 1 | 5.72 |
| CHEMBL474321 | MIGRASTATIN | — | 1 | - |
| CHEMBL1221965 | DORRIGOCIN B | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1221911 | LACTIMIDOMYCIN | IC50 | = | 37.82 | nM | 7.42 |
| CHEMBL1222014 | 2H LACTIMIDOMYCIN K | IC50 | = | 98.47 | nM | 7.01 |
| CHEMBL1554 | DACTINOMYCIN | IC50 | = | 519.7 | nM | 6.28 |
| CHEMBL123292 | CYCLOHEXIMIDE | IC50 | = | 532.5 | nM | 6.27 |
| CHEMBL1221912 | ISOMIGRASTATIN | IC50 | = | 624.0 | nM | 6.21 |
| CHEMBL1222015 | DIDEHYDRO ISOMIGRASTATIN | IC50 | = | 1909.0 | nM | 5.72 |
| CHEMBL123292 | CYCLOHEXIMIDE | Inhibition | - | % | - | |
| CHEMBL1554 | DACTINOMYCIN | Inhibition | - | % | - | |
| CHEMBL1221912 | ISOMIGRASTATIN | Inhibition | - | % | - | |
| CHEMBL1221911 | LACTIMIDOMYCIN | Inhibition | - | % | - | |
| CHEMBL474321 | MIGRASTATIN | Inhibition | - | % | - | |
| CHEMBL1221965 | DORRIGOCIN B | Inhibition | - | % | - |