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Assay Detail

CHEMBL1247347

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H](R)-alpha-methylhistamine from human histamine H3 receptor expressed in CHO cells by liquid scintillation counting
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Investigation of the histamine H3 receptor binding site. Design and synthesis of hybrid agonists with a lipophilic side chain.
Ishikawa M, Watanabe T, Kudo T, Yokoyama F, Yamauchi M, Kato K, Kakui N, Sato Y.
J Med Chem (2010) 53 : 6445 -6456
PubMed 20690643 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 17 7.47 9.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL18661 IMMEPIP 1 9.52
CHEMBL1098230 1 9.37
CHEMBL82298 IMMETHRIDINE 1 9.11
CHEMBL1243394 1 7.85
CHEMBL1243335 1 7.80
CHEMBL1243305 1 7.75
CHEMBL1243366 1 7.19
CHEMBL78838 1 7.12
CHEMBL1243334 1 7.12
CHEMBL1243365 1 6.92
CHEMBL1243393 1 6.89
CHEMBL1243395 1 6.75
CHEMBL1243367 1 6.70
CHEMBL1243333 1 6.28
CHEMBL81644 1 5.75
CHEMBL1243303 1 -
CHEMBL1243304 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL18661 IMMEPIP Ki = 0.3 nM 9.52
CHEMBL1098230 Ki = 0.43 nM 9.37
CHEMBL82298 IMMETHRIDINE Ki = 0.77 nM 9.11
CHEMBL1243394 Ki = 14.0 nM 7.85
CHEMBL1243335 Ki = 16.0 nM 7.80
CHEMBL1243305 Ki = 18.0 nM 7.75
CHEMBL1243366 Ki = 64.0 nM 7.19
CHEMBL78838 Ki = 76.0 nM 7.12
CHEMBL1243334 Ki = 75.0 nM 7.12
CHEMBL1243365 Ki = 120.0 nM 6.92
CHEMBL1243393 Ki = 130.0 nM 6.89
CHEMBL1243395 Ki = 180.0 nM 6.75
CHEMBL1243367 Ki = 200.0 nM 6.70
CHEMBL1243333 Ki = 530.0 nM 6.28
CHEMBL81644 Ki = 1800.0 nM 5.75
CHEMBL1243303 Ki > 1000.0 nM -
CHEMBL1243304 Ki > 1000.0 nM -