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Assay Detail

CHEMBL1248509

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]-4-DAMP from human muscarinic M3 receptor expressed in BHK-21 cells
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type BHK-21
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M3 (CHEMBL245)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of N-{1-[3-(3-oxo-2,3-dihydrobenzo[1,4]oxazin-4-yl)propyl]piperidin-4-yl}-2-phenylacetamide (Lu AE51090): an allosteric muscarinic M1 receptor agonist with unprecedented selectivity and procognitive potential.
Sams AG, Hentzer M, Mikkelsen GK, Larsen K, Bundgaard C, Plath N, Christoffersen CT, Bang-Andersen B.
J Med Chem (2010) 53 : 6386 -6397
PubMed 20684563 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 6 6.85 9.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL517712 ATROPINE 4.0 1 9.05
CHEMBL21536 XANOMELINE 4.0 1 7.89
CHEMBL845 N-DESMETHYLCLOZAPINE 1 7.05
CHEMBL522460 1 6.37
CHEMBL1242950 1 5.55
CHEMBL1242923 1 5.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL517712 ATROPINE Ki = 0.9 nM 9.05
CHEMBL21536 XANOMELINE Ki = 13.0 nM 7.89
CHEMBL845 N-DESMETHYLCLOZAPINE Ki = 90.0 nM 7.05
CHEMBL522460 Ki = 430.0 nM 6.37
CHEMBL1242950 Ki = 2800.0 nM 5.55
CHEMBL1242923 Ki = 7000.0 nM 5.16