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Assay Detail

CHEMBL1265709

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against recombinant HIV1 harboring reverse transcriptase V106A mutant clone infected in MAGIC-5 cells using 5-bromo4-chloro-3-indolyl-beta-D-galactopyranoside staining based light microscopy
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Human immunodeficiency virus 1
Cell Type MAGIC-5
Confidence 1 — Organism
Curated By Autocuration

Target

Human immunodeficiency virus 1 (CHEMBL378)
Type ORGANISM
Organism Human immunodeficiency virus 1

Publication

Combination of V106I and V179D polymorphic mutations in human immunodeficiency virus type 1 reverse transcriptase confers resistance to efavirenz and nevirapine but not etravirine.
Gatanaga H, Ode H, Hachiya A, Hayashida T, Sato H, Oka S.
Antimicrob Agents Chemother (2010) 54 : 1596 -1602
PubMed 20124001 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 3 7.76 9.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL308954 ETRAVIRINE 4.0 1 9.30
CHEMBL223228 EFAVIRENZ 4.0 1 8.52
CHEMBL57 NEVIRAPINE 4.0 1 5.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL308954 ETRAVIRINE EC50 = 0.5 nM 9.30
CHEMBL223228 EFAVIRENZ EC50 = 3.0 nM 8.52
CHEMBL57 NEVIRAPINE EC50 = 3430.0 nM 5.46