Assay Detail
Functional
CHEMBL1638840
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Agonist activity at human recombinant histamine H4 receptor expressed in Sf9 cells coexpressing RGS19, Galphai2, Gbeta1gamma2 by steady-state GTPase activity assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | Sf9 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Histamine H4 receptor agonists.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 8 | 6.91 | 8.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL471413 | — | — | 1 | 8.10 |
| CHEMBL90 | HISTAMINE | 4.0 | 1 | 7.90 |
| CHEMBL470790 | — | — | 1 | 7.80 |
| CHEMBL483406 | — | — | 1 | 7.80 |
| CHEMBL275443 | 4-METHYLHISTAMINE | — | 1 | 7.50 |
| CHEMBL24665 | — | — | 1 | 5.80 |
| CHEMBL12620 | 2-METHYLHISTAMINE | — | 1 | 5.40 |
| CHEMBL26116 | — | — | 1 | 5.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL471413 | — | EC50 | = | 7.943 | nM | 8.10 |
| CHEMBL90 | HISTAMINE | EC50 | = | 12.59 | nM | 7.90 |
| CHEMBL470790 | — | EC50 | = | 15.85 | nM | 7.80 |
| CHEMBL483406 | — | EC50 | = | 15.85 | nM | 7.80 |
| CHEMBL275443 | 4-METHYLHISTAMINE | EC50 | = | 31.62 | nM | 7.50 |
| CHEMBL24665 | — | EC50 | = | 1584.89 | nM | 5.80 |
| CHEMBL12620 | 2-METHYLHISTAMINE | EC50 | = | 3981.07 | nM | 5.40 |
| CHEMBL26116 | — | EC50 | = | 10000.0 | nM | 5.00 |