Assay Detail
Functional
CHEMBL1641107
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Induction of differentiation of human HL60 cells promyelocytes to monocytes after 4 days by NBT reduction assay
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | HL-60 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Removal of the 26-methyl group from 19-nor-1α,25-dihydroxyvitamin D₃ markedly reduces in vivo calcemic activity without altering in vitro VDR binding, HL-60 cell differentiation, and transcription.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| Ki | 14 | 8.74 | 10.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL605525 | DP001 | 2.0 | 1 | 10.10 |
| CHEMBL1630755 | — | — | 1 | 10.05 |
| CHEMBL1630759 | — | — | 1 | 10.00 |
| CHEMBL1630757 | — | — | 1 | 9.40 |
| CHEMBL3085140 | — | — | 1 | 9.22 |
| CHEMBL1630754 | — | — | 1 | 9.00 |
| CHEMBL846 | CALCITRIOL | 4.0 | 1 | 8.70 |
| CHEMBL3085139 | — | — | 1 | 8.70 |
| CHEMBL1630758 | — | — | 1 | 8.70 |
| CHEMBL1630756 | — | — | 1 | 8.30 |
| CHEMBL1630763 | — | — | 1 | 8.22 |
| CHEMBL1630761 | — | — | 1 | 7.70 |
| CHEMBL1630762 | — | — | 1 | 7.22 |
| CHEMBL1630760 | — | — | 1 | 7.00 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL605525 | DP001 | Ki | = | 0.08 | nM | 10.10 |
| CHEMBL1630755 | — | Ki | = | 0.09 | nM | 10.05 |
| CHEMBL1630759 | — | Ki | = | 0.1 | nM | 10.00 |
| CHEMBL1630757 | — | Ki | = | 0.4 | nM | 9.40 |
| CHEMBL3085140 | — | Ki | = | 0.6 | nM | 9.22 |
| CHEMBL1630754 | — | Ki | = | 1.0 | nM | 9.00 |
| CHEMBL846 | CALCITRIOL | Ki | = | 2.0 | nM | 8.70 |
| CHEMBL3085139 | — | Ki | = | 2.0 | nM | 8.70 |
| CHEMBL1630758 | — | Ki | = | 2.0 | nM | 8.70 |
| CHEMBL1630756 | — | Ki | = | 5.0 | nM | 8.30 |
| CHEMBL1630763 | — | Ki | = | 6.0 | nM | 8.22 |
| CHEMBL1630761 | — | Ki | = | 20.0 | nM | 7.70 |
| CHEMBL1630762 | — | Ki | = | 60.0 | nM | 7.22 |
| CHEMBL1630760 | — | Ki | = | 100.0 | nM | 7.00 |