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Assay Detail

CHEMBL1645651

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant CRTH2 receptor expressed in HEK293 cells assessed as inhibition of DK-PGD2-induced intracellular cAMP formation
37
Total Activities
37
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin D2 receptor 2 (CHEMBL5071)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of MK-7246, a selective CRTH2 antagonist for the treatment of respiratory diseases.
Gallant M, Beaulieu C, Berthelette C, Colucci J, Crackower MA, Dalton C, Denis D, Ducharme Y, Friesen RW, Guay D, Gervais FG, Hamel M, Houle R, Krawczyk CM, Kosjek B, Lau S, Leblanc Y, Lee EE, Levesque JF, Mellon C, Molinaro C, Mullet W, O'Neill GP, O'Shea P, Sawyer N, Sillaots S, Simard D, Slipetz D, Stocco R, Sørensen D, Truong VL, Wong E, Wu J, Zaghdane H, Wang Z.
Bioorg Med Chem Lett (2011) 21 : 288 -293
PubMed 21106375 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 37 7.86 8.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1643783 1 8.60
CHEMBL1643780 1 8.60
CHEMBL1643773 1 8.59
CHEMBL561132 MK-7246 1.0 1 8.52
CHEMBL1643771 1 8.52
CHEMBL1643774 1 8.52
CHEMBL1643775 1 8.44
CHEMBL1643779 1 8.42
CHEMBL1643778 1 8.35
CHEMBL1643787 1 8.31
CHEMBL1643798 1 8.26
CHEMBL1643789 1 8.24
CHEMBL1643782 1 8.24
CHEMBL1643788 1 8.17
CHEMBL1643790 1 8.16
CHEMBL1643786 1 8.09
CHEMBL1643796 1 8.07
CHEMBL1643776 1 8.05
CHEMBL1643784 1 8.00
CHEMBL1643795 1 7.92
CHEMBL1643801 1 7.89
CHEMBL1643794 1 7.89
CHEMBL1643799 1 7.82
CHEMBL1643791 1 7.66
CHEMBL1643781 1 7.62
CHEMBL1643800 1 7.60
CHEMBL1643770 1 7.58
CHEMBL1643792 1 7.57
CHEMBL1643793 1 7.48
CHEMBL1643785 1 7.45

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1643783 IC50 = 2.5 nM 8.60
CHEMBL1643780 IC50 = 2.5 nM 8.60
CHEMBL1643773 IC50 = 2.6 nM 8.59
CHEMBL561132 MK-7246 IC50 = 3.0 nM 8.52
CHEMBL1643771 IC50 = 3.0 nM 8.52
CHEMBL1643774 IC50 = 3.0 nM 8.52
CHEMBL1643775 IC50 = 3.6 nM 8.44
CHEMBL1643779 IC50 = 3.8 nM 8.42
CHEMBL1643778 IC50 = 4.5 nM 8.35
CHEMBL1643787 IC50 = 4.9 nM 8.31
CHEMBL1643798 IC50 = 5.5 nM 8.26
CHEMBL1643789 IC50 = 5.7 nM 8.24
CHEMBL1643782 IC50 = 5.7 nM 8.24
CHEMBL1643788 IC50 = 6.7 nM 8.17
CHEMBL1643790 IC50 = 6.9 nM 8.16
CHEMBL1643786 IC50 = 8.1 nM 8.09
CHEMBL1643796 IC50 = 8.5 nM 8.07
CHEMBL1643776 IC50 = 9.0 nM 8.05
CHEMBL1643784 IC50 = 9.9 nM 8.00
CHEMBL1643795 IC50 = 12.0 nM 7.92
CHEMBL1643801 IC50 = 13.0 nM 7.89
CHEMBL1643794 IC50 = 13.0 nM 7.89
CHEMBL1643799 IC50 = 15.0 nM 7.82
CHEMBL1643791 IC50 = 22.0 nM 7.66
CHEMBL1643781 IC50 = 24.0 nM 7.62
CHEMBL1643800 IC50 = 25.0 nM 7.60
CHEMBL1643770 IC50 = 26.5 nM 7.58
CHEMBL1643792 IC50 = 27.0 nM 7.57
CHEMBL1643793 IC50 = 33.0 nM 7.48
CHEMBL1643785 IC50 = 35.5 nM 7.45
CHEMBL1643777 IC50 = 38.0 nM 7.42
CHEMBL1643802 IC50 = 63.0 nM 7.20
CHEMBL1643797 IC50 = 214.0 nM 6.67
CHEMBL1643803 IC50 = 250.0 nM 6.60
CHEMBL1643804 IC50 = 308.0 nM 6.51
CHEMBL1643769 IC50 = 864.0 nM 6.06
CHEMBL1643772 CRTH2-negative-control IC50 > 10000.0 nM -