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Assay Detail

CHEMBL1656964

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of Stat5 phosphorylation in human HEL cells after 1 hr by Western blotting
1
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEL
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Signal transducer and activator of transcription 5A (CHEMBL5403)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 5-chloro-N2-[(1S)-1-(5-fluoropyrimidin-2-yl)ethyl]-N4-(5-methyl-1H-pyrazol-3-yl)pyrimidine-2,4-diamine (AZD1480) as a novel inhibitor of the Jak/Stat pathway.
Ioannidis S, Lamb ML, Wang T, Almeida L, Block MH, Davies AM, Peng B, Su M, Zhang HJ, Hoffmann E, Rivard C, Green I, Howard T, Pollard H, Read J, Alimzhanov M, Bebernitz G, Bell K, Ye M, Huszar D, Zinda M.
J Med Chem (2011) 54 : 262 -276
PubMed 21138246 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 1 7.39 7.39

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1231124 AZD-1480 2.0 1 7.39

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1231124 AZD-1480 IC50 = 41.0 nM 7.39