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Assay Detail

CHEMBL1659942

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiviral activity against HIV1 expressing protease L10I/G48V/I54V/A71V/I84V/L90M mutant infected in human MT4 cells selected at 5 uM of saquinavir by MTT assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Human immunodeficiency virus 1
Cell Type MT4
Confidence 1 — Organism
Curated By Autocuration

Target

Human immunodeficiency virus 1 (CHEMBL378)
Type ORGANISM
Organism Human immunodeficiency virus 1

Publication

Novel protease inhibitors (PIs) containing macrocyclic components and 3(R),3a(S),6a(R)-bis-tetrahydrofuranylurethane that are potent against multi-PI-resistant HIV-1 variants in vitro.
Tojo Y, Koh Y, Amano M, Aoki M, Das D, Kulkarni S, Anderson DD, Ghosh AK, Mitsuya H.
Antimicrob Agents Chemother (2010) 54 : 3460 -3470
PubMed 20439612 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 9 7.23 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1323 DARUNAVIR 4.0 1 8.52
CHEMBL571882 1 7.70
CHEMBL222559 TIPRANAVIR 4.0 1 7.66
CHEMBL577560 1 7.34
CHEMBL729 LOPINAVIR 4.0 1 6.92
CHEMBL116 AMPRENAVIR 4.0 1 6.75
CHEMBL1163 ATAZANAVIR 4.0 1 6.68
CHEMBL584 NELFINAVIR 4.0 1 6.29
CHEMBL114 SAQUINAVIR 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1323 DARUNAVIR EC50 = 3.0 nM 8.52
CHEMBL571882 EC50 = 20.0 nM 7.70
CHEMBL222559 TIPRANAVIR EC50 = 22.0 nM 7.66
CHEMBL577560 EC50 = 46.0 nM 7.34
CHEMBL729 LOPINAVIR EC50 = 120.0 nM 6.92
CHEMBL116 AMPRENAVIR EC50 = 180.0 nM 6.75
CHEMBL1163 ATAZANAVIR EC50 = 210.0 nM 6.68
CHEMBL584 NELFINAVIR EC50 = 510.0 nM 6.29
CHEMBL114 SAQUINAVIR EC50 > 1000.0 nM -