Assay Detail
Binding
CHEMBL1673550
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Activation of M3-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | HEK293 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Publication
FRET-based sensors for the human M1-, M3-, and M5-acetylcholine receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 6.03 | 6.66 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1184 | ACETYLCHOLINE CHLORIDE | 4.0 | 1 | 6.66 |
| CHEMBL1255785 | MUSCARINE CHLORIDE | — | 1 | 6.00 |
| CHEMBL23957 | OXOTREMORINE METHIODIDE | — | 1 | 5.75 |
| CHEMBL14 | CARBACHOL | 4.0 | 1 | 5.72 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1184 | ACETYLCHOLINE CHLORIDE | EC50 | = | 220.0 | nM | 6.66 |
| CHEMBL1255785 | MUSCARINE CHLORIDE | EC50 | = | 1000.0 | nM | 6.00 |
| CHEMBL23957 | OXOTREMORINE METHIODIDE | EC50 | = | 1800.0 | nM | 5.75 |
| CHEMBL14 | CARBACHOL | EC50 | = | 1900.0 | nM | 5.72 |