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Assay Detail

CHEMBL1681027

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of trimethoprim-resistant Staphylococcus aureus DfrB type dihydrofolate reductase F98Y mutant
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Staphylococcus aureus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dihydrofolate reductase (CHEMBL1681620)
Type SINGLE PROTEIN
Organism Staphylococcus aureus

Publication

In vitro and in vivo properties of dihydrophthalazine antifolates, a novel family of antibacterial drugs.
Caspers P, Bury L, Gaucher B, Heim J, Shapiro S, Siegrist S, Schmitt-Hoffmann A, Thenoz L, Urwyler H.
Antimicrob Agents Chemother (2009) 53 : 3620 -3627
PubMed 19546364 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 6.59 7.87

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1673173 1 7.87
CHEMBL1650306 1 7.65
CHEMBL1673175 1 7.39
CHEMBL1673177 1 7.19
CHEMBL1650307 1 7.15
CHEMBL1650308 1 6.91
CHEMBL1673174 1 5.94
CHEMBL1673176 1 5.54
CHEMBL1673178 1 5.24
CHEMBL22 TRIMETHOPRIM 4.0 1 4.99

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1673173 IC50 = 13.5 nM 7.87
CHEMBL1650306 IC50 = 22.6 nM 7.65
CHEMBL1673175 IC50 = 40.9 nM 7.39
CHEMBL1673177 IC50 = 64.4 nM 7.19
CHEMBL1650307 IC50 = 70.2 nM 7.15
CHEMBL1650308 IC50 = 124.4 nM 6.91
CHEMBL1673174 IC50 = 1146.0 nM 5.94
CHEMBL1673176 IC50 = 2917.0 nM 5.54
CHEMBL1673178 IC50 = 5705.0 nM 5.24
CHEMBL22 TRIMETHOPRIM IC50 = 10243.0 nM 4.99