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Assay Detail

CHEMBL1687498

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]flumazenil from GABAA receptor subunit alpha-2 expressed in CHO cells
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type CHO
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Gamma-aminobutyric acid receptor subunit alpha-2 (CHEMBL4956)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Exploring subtype selectivity and metabolic stability of a novel series of ligands for the benzodiazepine binding site of the GABAA receptor.
Hintermann S, Hurth K, Nozulak J, Tintelnot-Blomley M, Aichholz R, Blanz J, Kaupmann K, Mosbacher J.
Bioorg Med Chem Lett (2011) 21 : 1523 -1526
PubMed 21277199 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 17 6.84 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1681887 1 8.70
CHEMBL20042 CGS-9896 1 8.40
CHEMBL1681897 1 7.24
CHEMBL1681888 1 6.81
CHEMBL1681895 1 6.81
CHEMBL1681894 1 6.75
CHEMBL1681900 1 6.73
CHEMBL1681892 1 6.67
CHEMBL1681886 1 6.58
CHEMBL1681889 1 6.57
CHEMBL1681891 1 6.34
CHEMBL1681885 1 6.33
CHEMBL1681890 1 6.22
CHEMBL1681899 1 5.67
CHEMBL1681896 1 -
CHEMBL1681898 1 -
CHEMBL1681893 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1681887 IC50 = 2.0 nM 8.70
CHEMBL20042 CGS-9896 IC50 = 4.0 nM 8.40
CHEMBL1681897 IC50 = 57.0 nM 7.24
CHEMBL1681888 IC50 = 155.0 nM 6.81
CHEMBL1681895 IC50 = 154.0 nM 6.81
CHEMBL1681894 IC50 = 177.0 nM 6.75
CHEMBL1681900 IC50 = 185.0 nM 6.73
CHEMBL1681892 IC50 = 213.0 nM 6.67
CHEMBL1681886 IC50 = 265.0 nM 6.58
CHEMBL1681889 IC50 = 271.0 nM 6.57
CHEMBL1681891 IC50 = 461.0 nM 6.34
CHEMBL1681885 IC50 = 472.0 nM 6.33
CHEMBL1681890 IC50 = 605.0 nM 6.22
CHEMBL1681899 IC50 = 2114.0 nM 5.67
CHEMBL1681896 IC50 - -
CHEMBL1681898 IC50 > 3000.0 nM -
CHEMBL1681893 IC50 - -