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Assay Detail

CHEMBL1693145

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human H460 cells after 72 hrs by alamar blue assay in presence of 40 mg/ml HSA
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type NCI-H460
Confidence 1 — Organism
Curated By Autocuration

Target

NCI-H460 (CHEMBL396)
Type CELL-LINE
Organism Homo sapiens

Publication

14-Aminocamptothecins: their synthesis, preclinical activity, and potential use for cancer treatment.
Duan JX, Cai X, Meng F, Sun JD, Liu Q, Jung D, Jiao H, Matteucci J, Jung B, Bhupathi D, Ahluwalia D, Huang H, Hart CP, Matteucci M.
J Med Chem (2011) 54 : 1715 -1723
PubMed 21341674 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.09 7.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL481 IRINOTECAN 4.0 1 7.57
CHEMBL84 TOPOTECAN 4.0 1 6.96
CHEMBL1688857 1 6.92
CHEMBL1688855 1 6.92
CHEMBL65 CAMPTOTHECIN -1.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL481 IRINOTECAN IC50 = 27.0 nM 7.57
CHEMBL84 TOPOTECAN IC50 = 110.0 nM 6.96
CHEMBL1688857 IC50 = 120.0 nM 6.92
CHEMBL1688855 IC50 = 120.0 nM 6.92
CHEMBL65 CAMPTOTHECIN IC50 > 1000.0 nM -