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Assay Detail

CHEMBL1693237

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]DAMGO from mu opioid receptor in rat brain membrane homogenate by liquid scintillation counting
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL270)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Design, synthesis, pharmacological evaluation, and structure-activity study of novel endomorphin analogues with multiple structural modifications.
Mallareddy JR, Borics A, Keresztes A, Kövér KE, Tourwé D, Tóth G.
J Med Chem (2011) 54 : 1462 -1472
PubMed 21287991 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 16 8.46 9.89

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1689552 1 9.89
CHEMBL1689548 1 9.33
CHEMBL1689546 1 9.09
CHEMBL1689541 1 9.03
CHEMBL333357 ENDOMORPHIN 2 1 8.87
CHEMBL1689550 1 8.82
CHEMBL316446 ENDOMORPHIN-1 1 8.80
CHEMBL1689551 1 8.55
CHEMBL1689543 1 8.49
CHEMBL1689542 1 8.39
CHEMBL1689539 1 8.38
CHEMBL1689549 1 8.01
CHEMBL1689545 1 7.96
CHEMBL1689547 1 7.47
CHEMBL1689540 1 7.46
CHEMBL1689544 1 6.84

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1689552 Ki = 0.13 nM 9.89
CHEMBL1689548 Ki = 0.47 nM 9.33
CHEMBL1689546 Ki = 0.82 nM 9.09
CHEMBL1689541 Ki = 0.93 nM 9.03
CHEMBL333357 ENDOMORPHIN 2 Ki = 1.35 nM 8.87
CHEMBL1689550 Ki = 1.5 nM 8.82
CHEMBL316446 ENDOMORPHIN-1 Ki = 1.6 nM 8.80
CHEMBL1689551 Ki = 2.8 nM 8.55
CHEMBL1689543 Ki = 3.2 nM 8.49
CHEMBL1689542 Ki = 4.11 nM 8.39
CHEMBL1689539 Ki = 4.2 nM 8.38
CHEMBL1689549 Ki = 9.7 nM 8.01
CHEMBL1689545 Ki = 11.1 nM 7.96
CHEMBL1689547 Ki = 33.9 nM 7.47
CHEMBL1689540 Ki = 34.6 nM 7.46
CHEMBL1689544 Ki = 143.1 nM 6.84