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Assay Detail

CHEMBL1693471

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human 11beta-HSD2 expressed in HEK293 cells assessed as conversion of [1,2,6,7-[3H]cortisol to cortisone after 10 mins by scintillation counting
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

11-beta-hydroxysteroid dehydrogenase type 2 (CHEMBL3746)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of new glycyrrhetinic acid derived ring A azepanone, 29-urea and 29-hydroxamic acid derivatives as selective 11β-hydroxysteroid dehydrogenase 2 inhibitors.
Gaware R, Khunt R, Czollner L, Stanetty C, Da Cunha T, Kratschmar DV, Odermatt A, Kosma P, Jordis U, Classen-Houben D.
Bioorg Med Chem (2011) 19 : 1866 -1880
PubMed 21376605 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 7.39 8.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1269273 1 8.54
CHEMBL1689172 1 7.96
CHEMBL1689280 1 7.96
CHEMBL1689282 1 7.82
CHEMBL1689283 1 7.77
CHEMBL1689279 1 7.48
CHEMBL1689284 1 7.35
CHEMBL1689173 1 6.98
CHEMBL1689277 1 6.71
CHEMBL230006 ENOXOLONE 2.0 1 6.59
CHEMBL207413 ACETOXOLONE -1.0 1 6.12
CHEMBL1689281 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1269273 IC50 = 2.9 nM 8.54
CHEMBL1689172 IC50 = 11.0 nM 7.96
CHEMBL1689280 IC50 = 11.0 nM 7.96
CHEMBL1689282 IC50 = 15.0 nM 7.82
CHEMBL1689283 IC50 = 17.0 nM 7.77
CHEMBL1689279 IC50 = 33.0 nM 7.48
CHEMBL1689284 IC50 = 45.0 nM 7.35
CHEMBL1689173 IC50 = 104.0 nM 6.98
CHEMBL1689277 IC50 = 194.0 nM 6.71
CHEMBL230006 ENOXOLONE IC50 = 257.0 nM 6.59
CHEMBL207413 ACETOXOLONE IC50 = 750.0 nM 6.12
CHEMBL1689281 IC50 > 4000.0 nM -