Assay Detail
Functional
CHEMBL1694296
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at mouse melanocortin 5 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Mus musculus |
| Cell Type | HEK293 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Incorporation of a bioactive reverse-turn heterocycle into a peptide template using solid-phase synthesis to probe melanocortin receptor selectivity and ligand conformations by 2D 1H NMR.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 9 | 7.06 | 10.52 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL441738 | AFAMELANOTIDE | 4.0 | 1 | 10.52 |
| CHEMBL1688107 | — | — | 1 | 8.64 |
| CHEMBL1688110 | — | — | 1 | 7.14 |
| CHEMBL1688104 | — | — | 1 | 6.52 |
| CHEMBL1688108 | — | — | 1 | 6.47 |
| CHEMBL1688105 | — | — | 1 | 6.41 |
| CHEMBL1688111 | — | — | 1 | 6.03 |
| CHEMBL1688106 | — | — | 1 | 6.00 |
| CHEMBL1688109 | — | — | 1 | 5.80 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL441738 | AFAMELANOTIDE | EC50 | = | 0.03 | nM | 10.52 |
| CHEMBL1688107 | — | EC50 | = | 2.3 | nM | 8.64 |
| CHEMBL1688110 | — | EC50 | = | 72.0 | nM | 7.14 |
| CHEMBL1688104 | — | EC50 | = | 305.0 | nM | 6.52 |
| CHEMBL1688108 | — | EC50 | = | 335.0 | nM | 6.47 |
| CHEMBL1688105 | — | EC50 | = | 390.0 | nM | 6.41 |
| CHEMBL1688111 | — | EC50 | = | 925.0 | nM | 6.03 |
| CHEMBL1688106 | — | EC50 | = | 990.0 | nM | 6.00 |
| CHEMBL1688109 | — | EC50 | = | 1595.0 | nM | 5.80 |