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Assay Detail

CHEMBL1764806

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of staurosporine induced activation of caspase 3 activation in human Hela cells assessed as hydrolysis of Z-DEVD-R110 substrate by microplate fluorescence assay
27
Total Activities
27
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HeLa
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Caspase-3 (CHEMBL2334)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of isatin analogs as caspase-3 inhibitors: introduction of a hydrophilic group increases potency in a whole cell assay.
Chu W, Rothfuss J, Zhou D, Mach RH.
Bioorg Med Chem Lett (2011) 21 : 2192 -2197
PubMed 21441025 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 27 5.82 6.46

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1762363 1 6.46
CHEMBL1762349 1 6.42
CHEMBL1762353 1 6.41
CHEMBL1762362 1 6.38
CHEMBL372322 1 6.35
CHEMBL1762350 1 6.35
CHEMBL197549 1 6.19
CHEMBL456799 1 6.17
CHEMBL1762361 1 6.10
CHEMBL456798 1 6.03
CHEMBL1762366 1 5.97
CHEMBL1762352 1 5.89
CHEMBL1762364 1 5.86
CHEMBL1762351 1 5.77
CHEMBL371879 1 5.75
CHEMBL197535 1 5.74
CHEMBL1762346 1 5.70
CHEMBL1762347 1 5.67
CHEMBL1762365 1 5.60
CHEMBL200785 1 5.55
CHEMBL200813 1 5.51
CHEMBL371709 1 5.49
CHEMBL439753 1 5.43
CHEMBL213759 1 5.35
CHEMBL293034 1 5.12
CHEMBL200767 1 5.02
CHEMBL62993 1 4.94

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1762363 EC50 = 350.0 nM 6.46
CHEMBL1762349 EC50 = 380.0 nM 6.42
CHEMBL1762353 EC50 = 390.0 nM 6.41
CHEMBL1762362 EC50 = 420.0 nM 6.38
CHEMBL372322 EC50 = 450.0 nM 6.35
CHEMBL1762350 EC50 = 450.0 nM 6.35
CHEMBL197549 EC50 = 650.0 nM 6.19
CHEMBL456799 EC50 = 680.0 nM 6.17
CHEMBL1762361 EC50 = 800.0 nM 6.10
CHEMBL456798 EC50 = 930.0 nM 6.03
CHEMBL1762366 EC50 = 1060.0 nM 5.97
CHEMBL1762352 EC50 = 1300.0 nM 5.89
CHEMBL1762364 EC50 = 1390.0 nM 5.86
CHEMBL1762351 EC50 = 1690.0 nM 5.77
CHEMBL371879 EC50 = 1800.0 nM 5.75
CHEMBL197535 EC50 = 1830.0 nM 5.74
CHEMBL1762346 EC50 = 2000.0 nM 5.70
CHEMBL1762347 EC50 = 2150.0 nM 5.67
CHEMBL1762365 EC50 = 2500.0 nM 5.60
CHEMBL200785 EC50 = 2800.0 nM 5.55
CHEMBL200813 EC50 = 3070.0 nM 5.51
CHEMBL371709 EC50 = 3250.0 nM 5.49
CHEMBL439753 EC50 = 3670.0 nM 5.43
CHEMBL213759 EC50 = 4500.0 nM 5.35
CHEMBL293034 EC50 = 7670.0 nM 5.12
CHEMBL200767 EC50 = 9570.0 nM 5.02
CHEMBL62993 EC50 = 11500.0 nM 4.94