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Assay Detail

CHEMBL1768535

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Agonist activity at human GST-fused FXR LBD assessed as coactivator interaction with receptor ligand binding domain by Alphascreen assay
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Bile acid receptor (CHEMBL2047)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Extending SAR of bile acids as FXR ligands: discovery of 23-N-(carbocinnamyloxy)-3α,7α-dihydroxy-6α-ethyl-24-nor-5β-cholan-23-amine.
Gioiello A, Macchiarulo A, Carotti A, Filipponi P, Costantino G, Rizzo G, Adorini L, Pellicciari R.
Bioorg Med Chem (2011) 19 : 2650 -2658
PubMed 21459580 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 4 6.06 6.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1765565 1 6.82
CHEMBL566315 OBETICHOLIC ACID 4.0 1 6.70
CHEMBL3138092 1 6.05
CHEMBL240597 CHENODIOL 4.0 1 4.68

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1765565 EC50 = 150.0 nM 6.82
CHEMBL566315 OBETICHOLIC ACID EC50 = 200.0 nM 6.70
CHEMBL3138092 EC50 = 900.0 nM 6.05
CHEMBL240597 CHENODIOL EC50 = 21000.0 nM 4.68