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Assay Detail

CHEMBL1768754

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of mTOR in p53-deficient MEF assessed as phosphorylation of S6K1 at Thr389 by immunoblotting
26
Total Activities
26
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Serine/threonine-protein kinase mTOR (CHEMBL1255165)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Discovery of 9-(6-aminopyridin-3-yl)-1-(3-(trifluoromethyl)phenyl)benzo[h][1,6]naphthyridin-2(1H)-one (Torin2) as a potent, selective, and orally available mammalian target of rapamycin (mTOR) inhibitor for treatment of cancer.
Liu Q, Wang J, Kang SA, Thoreen CC, Hur W, Ahmed T, Sabatini DM, Gray NS.
J Med Chem (2011) 54 : 1473 -1480
PubMed 21322566 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 26 8.08 9.60

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1765602 1 9.60
CHEMBL1765605 1 9.00
CHEMBL1765606 1 9.00
CHEMBL1765608 1 9.00
CHEMBL1765707 1 9.00
CHEMBL1765708 1 9.00
CHEMBL1256459 TORIN1 1 8.70
CHEMBL1765615 1 8.70
CHEMBL1765709 1 8.70
CHEMBL1765710 1 8.30
CHEMBL1765609 1 8.00
CHEMBL1765603 1 8.00
CHEMBL1765610 1 8.00
CHEMBL1765616 1 8.00
CHEMBL1765614 1 8.00
CHEMBL1765612 1 7.70
CHEMBL1765613 1 7.70
CHEMBL1765601 1 7.60
CHEMBL1765607 1 7.60
CHEMBL1765712 1 7.60
CHEMBL1765714 1 7.46
CHEMBL1765604 1 7.30
CHEMBL1765611 1 7.30
CHEMBL1765706 1 7.30
CHEMBL1765711 1 7.00
CHEMBL1765713 1 6.60

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1765602 EC50 = 0.25 nM 9.60
CHEMBL1765605 EC50 = 1.0 nM 9.00
CHEMBL1765606 EC50 = 1.0 nM 9.00
CHEMBL1765608 EC50 = 1.0 nM 9.00
CHEMBL1765707 EC50 = 1.0 nM 9.00
CHEMBL1765708 EC50 = 1.0 nM 9.00
CHEMBL1256459 TORIN1 EC50 = 2.0 nM 8.70
CHEMBL1765615 EC50 = 2.0 nM 8.70
CHEMBL1765709 EC50 = 2.0 nM 8.70
CHEMBL1765710 EC50 = 5.0 nM 8.30
CHEMBL1765609 EC50 = 10.0 nM 8.00
CHEMBL1765603 EC50 = 10.0 nM 8.00
CHEMBL1765610 EC50 = 10.0 nM 8.00
CHEMBL1765616 EC50 = 10.0 nM 8.00
CHEMBL1765614 EC50 = 10.0 nM 8.00
CHEMBL1765612 EC50 = 20.0 nM 7.70
CHEMBL1765613 EC50 = 20.0 nM 7.70
CHEMBL1765601 EC50 = 25.0 nM 7.60
CHEMBL1765607 EC50 = 25.0 nM 7.60
CHEMBL1765712 EC50 = 25.0 nM 7.60
CHEMBL1765714 EC50 = 35.0 nM 7.46
CHEMBL1765604 EC50 = 50.0 nM 7.30
CHEMBL1765611 EC50 = 50.0 nM 7.30
CHEMBL1765706 EC50 = 50.0 nM 7.30
CHEMBL1765711 EC50 = 100.0 nM 7.00
CHEMBL1765713 EC50 = 250.0 nM 6.60