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Assay Detail

CHEMBL1769102

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant p38alpha assessed as incorporation of 33P from gamma-[33P]ATP into myelin basic protein after 30 mins by scintillation counting
35
Total Activities
35
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mitogen-activated protein kinase 14 (CHEMBL260)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of 6-(2,4-difluorophenoxy)-2-[3-hydroxy-1-(2-hydroxyethyl)propylamino]-8-methyl-8H-pyrido[2,3-d]pyrimidin-7-one (pamapimod) and 6-(2,4-difluorophenoxy)-8-methyl-2-(tetrahydro-2H-pyran-4-ylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (R1487) as orally bioavailable and highly selective inhibitors of p38α mitogen-activated protein kinase.
Goldstein DM, Soth M, Gabriel T, Dewdney N, Kuglstatter A, Arzeno H, Chen J, Bingenheimer W, Dalrymple SA, Dunn J, Farrell R, Frauchiger S, La Fargue J, Ghate M, Graves B, Hill RJ, Li F, Litman R, Loe B, McIntosh J, McWeeney D, Papp E, Park J, Reese HF, Roberts RT, Rotstein D, San Pablo B, Sarma K, Stahl M, Sung ML, Suttman RT, Sjogren EB, Tan Y, Trejo A, Welch M, Weller P, Wong BR, Zecic H.
J Med Chem (2011) 54 : 2255 -2265
PubMed 21375264 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 35 7.84 9.00

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1766572 1 9.00
CHEMBL1766498 1 9.00
CHEMBL1766579 1 9.00
CHEMBL1766503 1 9.00
CHEMBL1232784 1 8.70
CHEMBL1766500 1 8.70
CHEMBL1766506 1 8.70
CHEMBL1232772 1 8.40
CHEMBL1766580 1 8.40
CHEMBL1232769 1 8.30
CHEMBL1766502 1 8.22
CHEMBL1766505 1 8.15
CHEMBL1766499 1 8.05
CHEMBL1230122 R-1487 1.0 1 8.00
CHEMBL1230351 1 7.96
CHEMBL1766504 1 7.92
CHEMBL1766491 1 7.85
CHEMBL1090089 PAMAPIMOD 2.0 1 7.85
CHEMBL1766493 1 7.82
CHEMBL1766581 1 7.82
CHEMBL1766497 1 7.75
CHEMBL1766501 1 7.70
CHEMBL1766490 1 7.68
CHEMBL1766576 1 7.60
CHEMBL1766574 1 7.51
CHEMBL1766496 1 7.40
CHEMBL1766495 1 7.38
CHEMBL1230610 1 7.30
CHEMBL1766575 1 7.17
CHEMBL1232773 1 6.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1766572 IC50 = 1.0 nM 9.00
CHEMBL1766503 IC50 = 1.0 nM 9.00
CHEMBL1766579 IC50 = 1.0 nM 9.00
CHEMBL1766498 IC50 = 1.0 nM 9.00
CHEMBL1232784 IC50 = 2.0 nM 8.70
CHEMBL1766500 IC50 = 2.0 nM 8.70
CHEMBL1766506 IC50 = 2.0 nM 8.70
CHEMBL1232772 IC50 = 4.0 nM 8.40
CHEMBL1766580 IC50 = 4.0 nM 8.40
CHEMBL1232769 IC50 = 5.0 nM 8.30
CHEMBL1766502 IC50 = 6.0 nM 8.22
CHEMBL1766505 IC50 = 7.0 nM 8.15
CHEMBL1766499 IC50 = 9.0 nM 8.05
CHEMBL1230122 R-1487 IC50 = 10.0 nM 8.00
CHEMBL1230351 IC50 = 11.0 nM 7.96
CHEMBL1766504 IC50 = 12.0 nM 7.92
CHEMBL1090089 PAMAPIMOD IC50 = 14.0 nM 7.85
CHEMBL1766491 IC50 = 14.0 nM 7.85
CHEMBL1766493 IC50 = 15.0 nM 7.82
CHEMBL1766581 IC50 = 15.0 nM 7.82
CHEMBL1766497 IC50 = 18.0 nM 7.75
CHEMBL1766501 IC50 = 20.0 nM 7.70
CHEMBL1766490 IC50 = 21.0 nM 7.68
CHEMBL1766576 IC50 = 25.0 nM 7.60
CHEMBL1766574 IC50 = 31.0 nM 7.51
CHEMBL1766496 IC50 = 40.0 nM 7.40
CHEMBL1766495 IC50 = 42.0 nM 7.38
CHEMBL1230610 IC50 = 50.0 nM 7.30
CHEMBL1766575 IC50 = 67.0 nM 7.17
CHEMBL1232773 IC50 = 106.0 nM 6.97
CHEMBL1766494 IC50 = 111.0 nM 6.96
CHEMBL1766577 IC50 = 158.0 nM 6.80
CHEMBL1766578 IC50 = 223.0 nM 6.65
CHEMBL1766573 IC50 = 260.0 nM 6.58
CHEMBL1766492 IC50 = 594.0 nM 6.23