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Assay Detail

CHEMBL1769985

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human NET in HEK293 cells assessed as inhibition of [3H]norepinephrine uptake
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Sodium-dependent noradrenaline transporter (CHEMBL222)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis of 2-(substituted phenyl)-3,5,5-trimethylmorpholine analogues and their effects on monoamine uptake, nicotinic acetylcholine receptor function, and behavioral effects of nicotine.
Carroll FI, Muresan AZ, Blough BE, Navarro HA, Mascarella SW, Eaton JB, Huang X, Damaj MI, Lukas RJ.
J Med Chem (2011) 54 : 1441 -1448
PubMed 21319801 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 7.25 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1173597 1 8.05
CHEMBL1765600 1 7.89
CHEMBL1173772 1 7.72
CHEMBL1765599 1 7.62
CHEMBL1173275 1 7.50
CHEMBL1172928 RADAFAXINE 2.0 1 7.00
CHEMBL1173276 1 6.82
CHEMBL1171260 1 6.77
CHEMBL1172929 1 5.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1173597 IC50 = 9.0 nM 8.05
CHEMBL1765600 IC50 = 13.0 nM 7.89
CHEMBL1173772 IC50 = 19.0 nM 7.72
CHEMBL1765599 IC50 = 24.0 nM 7.62
CHEMBL1173275 IC50 = 32.0 nM 7.50
CHEMBL1172928 RADAFAXINE IC50 = 100.0 nM 7.00
CHEMBL1173276 IC50 = 150.0 nM 6.82
CHEMBL1171260 IC50 = 170.0 nM 6.77
CHEMBL1172929 IC50 = 1200.0 nM 5.92