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Assay Detail

CHEMBL1776269

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of fMLP/CB-activated human neutrophil degranulation assessed as inhibition of elastase release using MeO-Suc-Ala-Ala-Pro-Val-p-nitroanilide as a substrate after 5 mins
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Neutrophil
Confidence 1 — Organism
Curated By Autocuration

Target

Neutrophil (CHEMBL613710)
Type CELL-LINE

Publication

Bioactive constituents from the roots of Panax japonicus var. major and development of a LC-MS/MS method for distinguishing between natural and artifactual compounds.
Chan HH, Hwang TL, Reddy MV, Li DT, Qian K, Bastow KF, Lee KH, Wu TS.
J Nat Prod (2011) 74 : 796 -802
PubMed 21417387 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 5.24 6.12

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL168 OLEANOLIC_ACID 1 6.12
CHEMBL1773981 TAIBAIENOSIDE I 1 5.87
CHEMBL1773986 1 5.73
CHEMBL1773984 1 5.71
CHEMBL1095348 CHIKUSETSUSAPONIN IVA 1 5.32
CHEMBL502145 1 5.14
CHEMBL1288833 PSEUDOGINSENOSIDE RT1 METHYL ESTER 1 5.11
CHEMBL510111 1 5.02
CHEMBL1288834 PSEUDOGINSENOSIDE RT1 1 4.35
CHEMBL190503 PHENYLMETHYLSULFONYLFLUORIDE 1 4.02
CHEMBL365739 DIPHENYLENEIODONIUM 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL168 OLEANOLIC_ACID IC50 = 750.0 nM 6.12
CHEMBL1773981 TAIBAIENOSIDE I IC50 = 1360.0 nM 5.87
CHEMBL1773986 IC50 = 1870.0 nM 5.73
CHEMBL1773984 IC50 = 1970.0 nM 5.71
CHEMBL1095348 CHIKUSETSUSAPONIN IVA IC50 = 4760.0 nM 5.32
CHEMBL502145 IC50 = 7280.0 nM 5.14
CHEMBL1288833 PSEUDOGINSENOSIDE RT1 METHYL ESTER IC50 = 7750.0 nM 5.11
CHEMBL510111 IC50 = 9640.0 nM 5.02
CHEMBL1288834 PSEUDOGINSENOSIDE RT1 IC50 = 44500.0 nM 4.35
CHEMBL190503 PHENYLMETHYLSULFONYLFLUORIDE IC50 = 95000.0 nM 4.02
CHEMBL365739 DIPHENYLENEIODONIUM IC50 - -